PMID- 7988710
OWN - NLM
STAT- MEDLINE
DCOM- 19950111
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 356
IP  - 1
DP  - 1994 Dec 12
TI  - Histidine-419 plays a role in energy coupling in the vesicular monoamine 
      transporter from rat.
PG  - 145-50
AB  - Vesicular monoamine transporters (VMAT) catalyze transport of serotonin, 
      dopamine, epinephrine and norepinephrine into subcellular storage organelles in a 
      variety of cells. Accumulation of the neurotransmitter depends on the proton 
      electrochemical gradient across the organelle membrane and involves VMAT-mediated 
      exchange of two lumenal protons with one cytoplasmic amine. It has been suggested 
      in the past that His residues play a role in H+ movement or in its coupling to 
      active transport in H(+)-symporters and antiporters. Indeed VMAT-mediated 
      transport is inhibited by reagents specific for His residues. We have identified 
      one His residue in VMAT1 from rat which is conserved in other vesicular 
      neurotransmitter transporters. Mutagenesis of this His (H419) to either Arg or 
      Cys completely inhibits [3H]serotonin and [3H]dopamine accumulation. Mutagenesis 
      also inhibits other H(+)-dependent partial reactions of VMAT such as the 
      acceleration of binding of the high affinity ligand reserpine, but does not 
      inhibit the [3H]reserpine binding which is not dependent on H+ translocation. It 
      is concluded that His-419 plays a role in energy coupling in r-VMAT1.
FAU - Shirvan, A
AU  - Shirvan A
AD  - Alexander Silberman Institute of Life Sciences, Hebrew University, Jerusalem, 
      Israel.
FAU - Laskar, O
AU  - Laskar O
FAU - Steiner-Mordoch, S
AU  - Steiner-Mordoch S
FAU - Schuldiner, S
AU  - Schuldiner S
LA  - eng
GR  - NS16708/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Slc18a1 protein, rat)
RN  - 0 (Vesicular Biogenic Amine Transport Proteins)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - 333DO1RDJY (Serotonin)
RN  - 4QD397987E (Histidine)
RN  - 8B1QWR724A (Reserpine)
RN  - 94ZLA3W45F (Arginine)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Animals
MH  - Arginine/metabolism
MH  - Biological Transport
MH  - Cysteine/metabolism
MH  - Energy Metabolism
MH  - Glycoproteins/genetics/*metabolism
MH  - Histidine/*metabolism
MH  - *Membrane Glycoproteins
MH  - *Membrane Transport Proteins
MH  - Mutagenesis, Site-Directed
MH  - *Neuropeptides
MH  - Neurotransmitter Agents/genetics/*metabolism
MH  - Rats
MH  - Reserpine/metabolism
MH  - Serotonin/metabolism
MH  - Vesicular Biogenic Amine Transport Proteins
MH  - Vesicular Monoamine Transport Proteins
EDAT- 1994/12/12 00:00
MHDA- 1994/12/12 00:01
CRDT- 1994/12/12 00:00
PHST- 1994/12/12 00:00 [pubmed]
PHST- 1994/12/12 00:01 [medline]
PHST- 1994/12/12 00:00 [entrez]
AID - 0014-5793(94)01252-0 [pii]
AID - 10.1016/0014-5793(94)01252-0 [doi]
PST - ppublish
SO  - FEBS Lett. 1994 Dec 12;356(1):145-50. doi: 10.1016/0014-5793(94)01252-0.