PMID- 7989047
OWN - NLM
STAT- MEDLINE
DCOM- 19950110
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 94
IP  - 6
DP  - 1994 Dec
TI  - Male meiotic segregation of gonosomes analysed by two colour FISH in human 
      interphase spermatozoa.
PG  - 701-4
AB  - Human meiotic segregation of X and Y chromosomes was simultaneously analysed by 
      dual fluorescence in situ hybridization (FISH) on 10,638 interphase spermatozoa 
      from the same donor. A modified method for sperm decondensation ensured access of 
      both X and Y probes to the sperm chromatin and a 99% hybridization efficiency. 
      Expected sex ratios were obtained (49.30% haploidy X and 49.22% haploidy Y). The 
      frequencies of meiotic II non-disjunctions for X and Y chromosomes (0.05%) were 
      similar to those observed in sperm karyotypes after heterospecific fertilization 
      of hamster eggs. In contrast, the frequency of XY bearing cells was significantly 
      higher (0.42%). However, XY cells detected by FISH could either be diploid 
      somatic cells, diploid germinal cells or hyperhaploid XY spermatozoa, the latter 
      resulting from meiotic I non-disjunctions.
FAU - Chevret, E
AU  - Chevret E
AD  - Cytogenetic and Reproductive Biology Laboratory, Grenoble University Medical 
      School, France.
FAU - Rousseaux, S
AU  - Rousseaux S
FAU - Monteil, M
AU  - Monteil M
FAU - Cozzi, J
AU  - Cozzi J
FAU - Pelletier, R
AU  - Pelletier R
FAU - Mollard, J
AU  - Mollard J
FAU - Sele, B
AU  - Sele B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosome Aberrations
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interphase/genetics
MH  - Male
MH  - Meiosis/*genetics
MH  - Sex Chromosomes/*genetics
MH  - Spermatozoa/*chemistry
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1007/BF00206967 [doi]
PST - ppublish
SO  - Hum Genet. 1994 Dec;94(6):701-4. doi: 10.1007/BF00206967.