PMID- 7989454
OWN - NLM
STAT- MEDLINE
DCOM- 19950112
LR  - 20161123
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 79
IP  - 6
DP  - 1994 Dec
TI  - Circulating fibroblast growth factor-like autoantibodies in two patients with 
      multiple endocrine neoplasia type 1 and prolactinoma.
PG  - 1546-52
AB  - Basic fibroblast growth factor (bFGF) is a potent endothelial cell mitogen found 
      in a variety of normal and tumor tissues. bFGF lacks a classical amino-terminal 
      signal sequence and is not readily detectable in plasma from normal subjects. In 
      earlier studies we showed increased bFGF-like mitogenic activity for 
      parathyroid-derived endothelial cells and (increased) bFGF immunoreactivity 
      (0.24-1.28 ng/mL) in plasma of subjects with multiple endocrine neoplasia type 1 
      (MEN-1). In the present study we examined the proliferative activity of MEN-1 and 
      normal plasmas (applied to protein-A columns) in calf pulmonary artery 
      endothelial cells. Protein-A-eluted activity in plasma from MEN-1 prolactinoma 
      plasma exceeded activity from normal and MEN-1 nonprolactinoma plasma in three of 
      eight MEN-1 subjects with untreated or recurrent prolactinoma. Protein-A-eluted 
      active fractions from MEN-1 prolactinoma plasma had several properties of an 
      immunoglobulin G, including affinity for antihuman immunoglobulin G (IgG) 
      agarose, sensitivity to thiols, and (prepared by sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis under reducing conditions) apparent 
      mol wt corresponding to those of the heavy and light chains of IgG. The IgG 
      fraction of MEN-1 prolactinoma plasma had far more activity in endothelial cells 
      than did optimal concentrations of known growth factors or conditioned medium 
      from prolactinoma cells. Endothelial cell bioactivity in protein-A-eluted 
      fractions from MEN-1 prolactinoma plasma was neutralized 70% by rabbit antibodies 
      to intact bFGF. These results imply novel growth stimulatory bFGF-like 
      autoantibodies in a subset of MEN-1 patients with prolactinoma.
FAU - Zimering, M B
AU  - Zimering MB
AD  - Veterans Affairs Medical Center, Lyons, New Jersey 07939.
FAU - Riley, D J
AU  - Riley DJ
FAU - Thakker-Varia, S
AU  - Thakker-Varia S
FAU - Walker, A M
AU  - Walker AM
FAU - Lakshminaryan, V
AU  - Lakshminaryan V
FAU - Shah, R
AU  - Shah R
FAU - Brandi, M L
AU  - Brandi ML
FAU - Ezzat, S
AU  - Ezzat S
FAU - Katsumata, N
AU  - Katsumata N
FAU - Friesen, H G
AU  - Friesen HG
AU  - et al.
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Autoantibodies)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Staphylococcal Protein A)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Autoantibodies/*blood
MH  - Cattle
MH  - Cell Division/drug effects
MH  - Chemical Phenomena
MH  - Chemistry, Physical
MH  - Chromatography, Affinity
MH  - Endothelium, Vascular/*cytology
MH  - Female
MH  - Fibroblast Growth Factor 2/*immunology
MH  - Humans
MH  - Immunoglobulin G/blood/chemistry/pharmacology
MH  - Male
MH  - Middle Aged
MH  - Multiple Endocrine Neoplasia/*immunology
MH  - Pituitary Neoplasms/*immunology
MH  - Prolactinoma/*immunology
MH  - Rats
MH  - Staphylococcal Protein A
MH  - Tumor Cells, Cultured
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1210/jcem.79.6.7989454 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 1994 Dec;79(6):1546-52. doi: 10.1210/jcem.79.6.7989454.