PMID- 7992895
OWN - NLM
STAT- MEDLINE
DCOM- 19950112
LR  - 20061115
IS  - 0003-276X (Print)
IS  - 0003-276X (Linking)
VI  - 240
IP  - 2
DP  - 1994 Oct
TI  - Structural, morphometric, and immunohistological study of the accessory olfactory 
      bulb in the dog.
PG  - 277-85
AB  - BACKGROUND: The study of the morphological, morphometric, and immunohistological 
      characteristics of the accessory olfactory bulb (AOB) in the dog is the main goal 
      of this work. METHODS: Horizontal sections of the AOB where stained by four 
      different methods (haematoxilin/eosin, Tolivia, Nissl, and Bielchowsky). The 
      avidin-biotin-peroxidase complex (ABC) was used, whereas the monoclonal 
      antibodies to neuron-specific enolase, neurofilaments, glial fibrillary acidic 
      protein, and synaptophysin were selected for the immunohistological study. A 
      computer-assisted image analysis was employed in order to define the morphometric 
      characteristics of de AOB. RESULTS: The general morphology of the AOB indicates 
      that it comprises a thick glomerular layer and a thinner internal layer 
      containing mitral/tufted, granular, and glial cells. The mitral/tufted cells have 
      large pale-staining nuclei with intensely staining nucleoli. There does not 
      appear to be a clearly defined granular layer. No reactivity with antibodies to 
      neuron-specific enolase or to neurofilaments was observed in any part of the AOB, 
      but there was some reactivity with an antibody to glial fibrillary acidic protein 
      and widespread reactivity with an antibody to synaptophysin. CONCLUSIONS: The 
      stratification of the AOB is simpler and less well defined than that of the main 
      olfactory bulb (MOB), unlike in rodents in which the structure of the AOB 
      corresponds closely to that of the MOB. According to the scale of Frahm and 
      Bhatnagar (1980. J. Anat., 130: 349-365) the AOB of the adult dog has an 
      intermediate position.
FAU - Salazar, I
AU  - Salazar I
AD  - Departamento de Anatomia y Embriologia, Facultad de Veterinaria de Lugo, 
      Universidad de Santiago de Compostela, Spain.
FAU - Cifuentes, J M
AU  - Cifuentes JM
FAU - Sanchez Quinteiro, P
AU  - Sanchez Quinteiro P
FAU - Garcia Caballero, T
AU  - Garcia Caballero T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anat Rec
JT  - The Anatomical record
JID - 0370540
SB  - IM
MH  - Animals
MH  - Dogs/*anatomy & histology
MH  - Female
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Olfactory Bulb/*anatomy & histology/chemistry/cytology
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1002/ar.1092400216 [doi]
PST - ppublish
SO  - Anat Rec. 1994 Oct;240(2):277-85. doi: 10.1002/ar.1092400216.