PMID- 7994385
OWN - NLM
STAT- MEDLINE
DCOM- 19950117
LR  - 20191101
IS  - 1073-1199 (Print)
IS  - 1073-1199 (Linking)
VI  - 22
IP  - 3
DP  - 1994
TI  - Application of aqueous two-phase systems in separation/purification of stroma 
      free hemoglobin from animal blood.
PG  - 641-9
AB  - Aqueous two-phase system (ATPS) is suitable for treatment of biological 
      materials. It has been traditionally employed in the separation of product from 
      fermentation broth, and in the extraction of large-molecule proteins from colloid 
      solution. The separation method based on ATPS features simple, rapid and 
      continuous operation with low equipment cost. A PEG/Salt aqueous two-phase system 
      is studied, and developed for hemoglobin (Hb) separation from animal bloods. 
      Stroma free hemoglobin (SFHb) was extracted from 200ml packed red blood cells by 
      the two-stage aqueous two-phase system and subsequently was desalted and dialyzed 
      by ultrafiltration. It took only 2.5hr to complete overall process to purify 
      SFHb. The purity of SFHb was analyzed by HPLC and IEF (silver staining). Large 
      partition coefficients of Hb have been observed with proper selection of PEG 
      molecular weight, added concentrations of PEG and salt in each phase. The SFHb of 
      very high purity which is free of other proteins (< 0.1% impurity) and 
      phospholipids (< 2% impurities) may be obtained from human packed RBCs. A 
      two-stage extraction design using ATPS separation concept is very simple and 
      useful for purification of SFHb. The method may be further developed to offer 
      potent technology for hemoglobin separation from animal blood and future 
      production industry of blood substitutes.
FAU - Kan, P
AU  - Kan P
AD  - Department of Chemical Engineering, National Tsing Hua University, Hsinchu, 
      Taiwan, ROC.
FAU - Lee, C J
AU  - Lee CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Artif Cells Blood Substit Immobil Biotechnol
JT  - Artificial cells, blood substitutes, and immobilization biotechnology
JID - 9431307
RN  - 0 (Blood Substitutes)
RN  - 0 (Hemoglobins)
RN  - 0 (Phosphates)
RN  - 0 (hemoglobin, stroma free)
RN  - 059QF0KO0R (Water)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Animals
MH  - Biotechnology
MH  - Blood Substitutes/*isolation & purification
MH  - Centrifugation
MH  - Chromatography, High Pressure Liquid
MH  - Hemoglobins/*isolation & purification
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Isoelectric Focusing
MH  - Phosphates
MH  - Polyethylene Glycols
MH  - Ultrafiltration
MH  - Water
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.3109/10731199409117894 [doi]
PST - ppublish
SO  - Artif Cells Blood Substit Immobil Biotechnol. 1994;22(3):641-9. doi: 
      10.3109/10731199409117894.