PMID- 7994930
OWN - NLM
STAT- MEDLINE
DCOM- 19950117
LR  - 20191210
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 74
IP  - 1
DP  - 1995 Jan
TI  - Human monocytes produce monocyte chemoattractant protein 1 (MCP-1) in response to 
      a synthetic peptide derived from C-reactive protein.
PG  - 84-8
AB  - We reported previously that a synthetic peptide (RS-83277) derived from human 
      C-reactive protein (CRP) augmented human monocyte/macrophage tumoricidal activity 
      and cytokine production. RS-83287, a synthetic peptide derived from a different 
      CRP site, was ineffective. Because chemoattractant properties have been 
      attributed to some CRP-derived peptides, we hypothesized that RS-83277, in 
      addition to activating effects, might promote human monocyte chemotaxis. Results 
      indicated that neither CRP peptide RS-83277 nor RS-83287 was, itself, a 
      chemoattractant. RS-83277, but not RS-83287, however, elicited time-dependent 
      production of monocyte chemoattractant activity in conditioned media (CM) of 
      cultured human mononuclear leukocytes and purified, adherent monocytes (MO). CM 
      from nonadherent MO contained no activity, indicating that adherence was required 
      for monocyte response. Monocyte chemoattractant activity was dose-dependent and 
      was removed by treatment with immobilized antibody to human monocyte 
      chemoattractant protein 1 (MCP-1) but not by irrelevant IgG. These results 
      indicate that a specific peptide segment of CRP acts upon human adherent 
      monocytes to promote production of the autocrine chemotactic and activating 
      factor MCP-1. Data suggest that degraded CRP represents a complex source of 
      biologically active peptides which, among other effects, may amplify monocyte 
      recruitment to sites of injury.
FAU - Zhou, P
AU  - Zhou P
AD  - Laboratory of Clinical Investigation, NIAID Branch, National Institutes of 
      Health, Bethesda, Maryland 20814.
FAU - Thomassen, M J
AU  - Thomassen MJ
FAU - Pettay, J
AU  - Pettay J
FAU - Deodhar, S D
AU  - Deodhar SD
FAU - Barna, B P
AU  - Barna BP
LA  - eng
GR  - CA-49950/CA/NCI NIH HHS/United States
GR  - CA-54248/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Peptides)
RN  - 0 (RS 83277)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - C-Reactive Protein/*chemistry
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*biosynthesis/chemistry/metabolism
MH  - Chemotaxis
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins
MH  - Monocytes/*metabolism/physiology
MH  - Peptides/*pharmacology
MH  - Sepharose
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - S0090122985710124 [pii]
AID - 10.1006/clin.1995.1012 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1995 Jan;74(1):84-8. doi: 10.1006/clin.1995.1012.