PMID- 7996851
OWN - NLM
STAT- MEDLINE
DCOM- 19950113
LR  - 20071114
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 57
IP  - 6
DP  - 1994 Dec
TI  - The expression of regenerative growth factors in chronic liver injury and repair.
PG  - 711-7
AB  - Acute hepatic injury initiates known cellular and molecular events for 
      regeneration. In contrast, the molecular mechanisms of repair following chronic 
      liver injuries have not been defined. Transforming growth factor alpha (TGF 
      alpha) and hepatocyte growth factor (HGF) are hepatocyte mitogens whose in vivo 
      expression in liver is central to the regulation of regeneration. To study the 
      role of TGF alpha and HGF in liver injury and repair, we used a model of 
      reversible biliary obstruction without a bilioenteric anastomosis. In rats, the 
      common bile duct was obstructed either by a vessel loop suspended from the 
      abdominal wall (LOOP) or by ligation and division (DLD). After 7 days of 
      obstruction, animals were autopsied or were decompressed by subcutaneous release 
      of the loop and then autopsied at 1, 2, 4, 7, or 10 days of postdecompression. 
      Serum bilirubin (mg/dl) increased to 14.8 +/- 2.9 (DLD) and 10.3 +/- 3.0 (LOOP) 
      (+/- SEM, NS, ANOVA) at 7 days of obstruction. Liver sections demonstrated equal 
      ductal hyperplasia and collagen deposition after LOOP and DLD. Biliary 
      decompression reversed bile duct proliferation and normalized bilirubin. Analysis 
      of injured and repairing liver mRNA by ribonuclease protection assay showed that 
      TGF alpha mRNA levels were not significantly altered by injury or during repair. 
      HGF mRNA was elevated following obstruction and showed increased expression 1 day 
      after decompression, peaking at 2 days of repair. This evidence of modulation of 
      HGF during liver repair following chronic cholestatic injury suggests that HGF 
      may have a role in cellular proliferation during repair or act as a compensatory 
      growth factor during injury.
FAU - Aldana, P R
AU  - Aldana PR
AD  - Department of Surgery, St. Louis University School of Medicine, Missouri.
FAU - Goerke, M E
AU  - Goerke ME
FAU - Carr, S C
AU  - Carr SC
FAU - Tracy, T F Jr
AU  - Tracy TF Jr
LA  - eng
GR  - DK 46831/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cell Division/physiology
MH  - Chronic Disease
MH  - Collagen/metabolism
MH  - Hepatocyte Growth Factor/analysis/genetics/*physiology
MH  - Liver/chemistry/pathology/physiology
MH  - Liver Diseases/metabolism/pathology/*physiopathology
MH  - Liver Regeneration/*physiology
MH  - Male
MH  - RNA, Messenger/analysis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor alpha/analysis/genetics/*physiology
EDAT- 1994/12/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - S0022-4804(84)71206-6 [pii]
AID - 10.1006/jsre.1994.1206 [doi]
PST - ppublish
SO  - J Surg Res. 1994 Dec;57(6):711-7. doi: 10.1006/jsre.1994.1206.