PMID- 7999125
OWN - NLM
STAT- MEDLINE
DCOM- 19950117
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 205
IP  - 1
DP  - 1994 Nov 30
TI  - Effect of cysteine substitutions on the mitogenic activity and stability of 
      recombinant human keratinocyte growth factor.
PG  - 872-9
AB  - Human Keratinocyte growth factor (hKGF), a member of the FGF family of growth 
      factors, contains five cysteines at amino acid positions 1, 15, 40, 102, and 106. 
      We expressed five cysteine mutants of hKGF in which the cysteines were 
      cumulatively replaced with alanine or serine, starting with cysteine-1. 
      Recombinant hKGF has an inherently higher mitogenic activity and stability to 
      heat and acid than reported for glycosylated hKGF. Mitogenic activity is 
      increased an additional 2.6 fold by substitution of cysteine-1 with alanine. 
      Mutants with the conserved cysteine substituted at position 40 were more 
      susceptible to heat inactivation than rhKGF, but showed no significant difference 
      in acid inactivation. Cysteine-free rhKGF is mitogenic, demonstrating that 
      neither cysteines nor disulfide bonds are required for mitogenic activity. 
      However, cysteine-free rhKGF does not bind Heparin-Sepharose and is unstable to 
      heat and acid compared to rhKGF, suggesting that the cysteines have a role in 
      maintaining KGF's structure. This information will useful in the development of a 
      more stable and more potent wound healing agent from hKGF.
FAU - Bare, L A
AU  - Bare LA
AD  - Ohmeda, PPD, Murray Hill, NJ 07974.
FAU - Brown, M
AU  - Brown M
FAU - Goyal, S
AU  - Goyal S
FAU - Idler, D
AU  - Idler D
FAU - Mansson, P E
AU  - Mansson PE
LA  - eng
SI  - GENBANK/U01670
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (FGF7 protein, human)
RN  - 0 (Fgf7 protein, mouse)
RN  - 0 (Fibroblast Growth Factor 10)
RN  - 0 (Growth Substances)
RN  - 0 (Mitogens)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Recombinant Proteins)
RN  - 126469-10-1 (Fibroblast Growth Factor 7)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cysteine/*genetics
MH  - Fibroblast Growth Factor 10
MH  - Fibroblast Growth Factor 7
MH  - *Fibroblast Growth Factors
MH  - Growth Substances/genetics/*pharmacology
MH  - Hot Temperature
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Keratinocytes/cytology/drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mitogens/*pharmacology
MH  - Molecular Sequence Data
MH  - Oligodeoxyribonucleotides
MH  - Recombinant Proteins/genetics/pharmacology
EDAT- 1994/11/30 00:00
MHDA- 1994/11/30 00:01
CRDT- 1994/11/30 00:00
PHST- 1994/11/30 00:00 [pubmed]
PHST- 1994/11/30 00:01 [medline]
PHST- 1994/11/30 00:00 [entrez]
AID - S0006-291X(84)72745-8 [pii]
AID - 10.1006/bbrc.1994.2745 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1994 Nov 30;205(1):872-9. doi: 
      10.1006/bbrc.1994.2745.