PMID- 7999672
OWN - NLM
STAT- MEDLINE
DCOM- 19950124
LR  - 20211203
IS  - 0923-1811 (Print)
IS  - 0923-1811 (Linking)
VI  - 7 Suppl
DP  - 1994 Jul
TI  - Modulation of the differentiated phenotype of keratinocytes of the hair follicle 
      and from epidermis.
PG  - S142-51
AB  - The differentiation capacity and its modulation by cell-cell and cell-matrix 
      interactions of epithelial cells from epidermis (NEK), hair follicle outer root 
      sheath (ORS) and hair matrix cells (HMC) in different experimental model systems 
      is reviewed. Reformation of structurally intact and functioning hair follicles 
      has been achieved with isolated neonatal mouse cells in vivo when follicular 
      epithelia and dermal fibroblasts were transplanted. This structural 
      reorganization has not yet been feasible in vitro in either surface or matrix 
      embedded organotypic cocultures with mesenchymal cells. While the epithelial 
      cells isolated from epidermis or hair follicle compartments formed similar 
      stratified and keratinizing epithelia in vitro, their degree of differentiation 
      was significantly different, declining from NEK over ORS to HMC. Differentiation 
      was further reduced in HMC cultures in the presence of dermal papilla cells 
      (DPC). Differentiation was analyzed by morphologic criteria and the biochemical 
      analysis as well as immunohistochemical localization of differentiation products 
      such as keratins, involucrin, filaggrin, integrins, basement membrane components 
      and membrane antigens. The results demonstrate fully maintained differentiation 
      capacity of skin and appendage-keratinocytes to reconstitute a squamous 
      epithelium, reflecting their common origin from embryonic epidermis. For the 
      induction of hair follicle-specific structural and functional characteristics, 
      different and probably more complex interactive mechanisms may be required.
FAU - Fusenig, N E
AU  - Fusenig NE
AD  - German Cancer Research Center (DKFZ), Division of Differentiation and 
      Carcinogenesis, Heidelberg.
FAU - Limat, A
AU  - Limat A
FAU - Stark, H J
AU  - Stark HJ
FAU - Breitkreutz, D
AU  - Breitkreutz D
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
SB  - IM
MH  - Animals
MH  - Cell Communication
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - *Epidermal Cells
MH  - Epithelial Cells
MH  - Filaggrin Proteins
MH  - Hair/*cytology/growth & development/transplantation
MH  - Humans
MH  - Keratinocytes/*cytology/transplantation
MH  - Mice
MH  - Mice, Nude
MH  - Organ Culture Techniques
MH  - Phenotype
MH  - Transplantation, Heterologous
RF  - 48
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 0923-1811(94)90045-0 [pii]
AID - 10.1016/0923-1811(94)90045-0 [doi]
PST - ppublish
SO  - J Dermatol Sci. 1994 Jul;7 Suppl:S142-51. doi: 10.1016/0923-1811(94)90045-0.