PMID- 7999678
OWN - NLM
STAT- MEDLINE
DCOM- 19950124
LR  - 20240109
IS  - 0923-1811 (Print)
IS  - 0923-1811 (Linking)
VI  - 7 Suppl
DP  - 1994 Jul
TI  - Dermal papilla cells express hepatocyte growth factor.
PG  - S79-83
AB  - In the induction, development and maintenance of hair follicles, it is thought 
      that an epithelial-mesenchymal interaction is important and that the dermal 
      papilla plays some important roles. Hepatocyte growth factor is a multifunctional 
      polypeptide which acts as mitogen, motogen or morphogen depending on the 
      biological context. Recently, we found that HGF stimulates hair follicle growth 
      in a mouse organ culture system, and therefore proceeded to investigate the 
      expression of HGF on cultured human dermal papilla cells (DPC) and the effect of 
      HGF on cultured human keratinocytes derived from hair bulb. Using an enzyme 
      immuno assay, HGF immunoreactivities were not detected in conditioned media of 
      DPC that were either non-treated or treated with TGF-beta, but were detected in 
      conditioned media of DPC treated with IL1-alpha, TNF-alpha and TPA. Using the 
      reverse transcription-polymerase chain reaction (RT-PCR) method, HGF mRNA was 
      also detected in DPC. This expression was enhanced by IL1-alpha, TNF-alpha and 
      TPA, but suppressed by TGF-beta. Furthermore, HGF stimulated the DNA synthesis in 
      keratinocytes derived from human hair bulb in a dose-dependent manner. These 
      results indicate that DPC express HGF in vitro and that HGF stimulates the growth 
      of human keratinocytes derived from hair bulb in vitro.
FAU - Shimaoka, S
AU  - Shimaoka S
AD  - Department of Dermatology, Juntendo University, School of Medicine, Tokyo, Japan.
FAU - Imai, R
AU  - Imai R
FAU - Ogawa, H
AU  - Ogawa H
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-1)
RN  - 0 (Peptide Fragments)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130349-12-1 (glyceraldehyde 3-phosphate dehydrogenase (304-313))
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
GS  - GAPDH
GS  - HGF
MH  - Animals
MH  - Base Sequence
MH  - Cell Division/drug effects
MH  - DNA Primers/genetics
MH  - Gene Expression/drug effects
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
MH  - Hair/*cytology/drug effects/*metabolism
MH  - Hepatocyte Growth Factor/genetics/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Interleukin-1/pharmacology
MH  - Keratinocytes/drug effects/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptide Fragments/genetics
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transforming Growth Factor beta/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 0923-1811(94)90038-8 [pii]
AID - 10.1016/0923-1811(94)90038-8 [doi]
PST - ppublish
SO  - J Dermatol Sci. 1994 Jul;7 Suppl:S79-83. doi: 10.1016/0923-1811(94)90038-8.