PMID- 7999680
OWN - NLM
STAT- MEDLINE
DCOM- 19950124
LR  - 20191023
IS  - 0923-1811 (Print)
IS  - 0923-1811 (Linking)
VI  - 7 Suppl
DP  - 1994 Jul
TI  - Mechanism of action of androgen in hair follicles.
PG  - S98-103
AB  - In order to investigate the mode of action of testosterone (T) on human hair 
      follicles we studied the metabolism of T and localization of androgen receptors 
      in outer root sheath cells (ORSC) and dermal papilla cells (DPC) from different 
      body sites. T was principally metabolized to androstenedione (delta 4) even in 
      beard ORSC as well as epidermal keratinocytes (EK), and the ratio of apparent 5 
      alpha-reductase (5 alpha-R) to 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) 
      did not differ between these two kinds of cells. The 5 alpha-R activity in beard 
      DPC was 3 times as high as that in occipital scalp and axillary DPC. The 5 
      alpha-R of beard DPC exhibited a narrow optimum pH of 5.5, which is 
      characteristic of type 2 enzyme present in androgen target cells. In contrast, 5 
      alpha-R of DPC from axillary and occipital scalp hair showed a broad optimum pH 
      range between 6.5-9.0 corresponding to type 1 5 alpha-R. Androgen receptors were 
      detected in the DPC of beard and axillary hair follicles, but not in those of 
      occipital scalp hair follicles using immunohistochemical staining with polyclonal 
      anti-androgen receptor antibody. Epithelial cells of the hair bulb were not 
      stained by the antibody. Androgen receptors were also detected in the nuclei of 
      cultured beard and axillary DPC, but the DPC from occipital scalp hair follicles 
      showed little staining with the antibody. We also examined the effects of T on 
      the DNA synthesis and proliferation of cultured ORSC and DPC. T did not have a 
      proliferative effect on either type of cell when cultured alone.(ABSTRACT 
      TRUNCATED AT 250 WORDS)
FAU - Itami, S
AU  - Itami S
AD  - Department of Dermatology, Oita Medical University, Japan.
FAU - Sonoda, T
AU  - Sonoda T
FAU - Kurata, S
AU  - Kurata S
FAU - Takayasu, S
AU  - Takayasu S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (Receptors, Androgen)
RN  - 3XMK78S47O (Testosterone)
RN  - 409J2J96VR (Androstenedione)
RN  - 4KM2BN5JHF (Cyproterone Acetate)
RN  - 9007-49-2 (DNA)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
RN  - EC 1.3.1.22 (Cholestenone 5 alpha-Reductase)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/metabolism
MH  - Androstenedione/metabolism
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Cholestenone 5 alpha-Reductase
MH  - Cyproterone Acetate/pharmacology
MH  - DNA/biosynthesis
MH  - Hair/cytology/*drug effects/metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Immunohistochemistry
MH  - Keratinocytes/metabolism
MH  - Male
MH  - Oxidoreductases/metabolism
MH  - Receptors, Androgen/metabolism
MH  - Testosterone/metabolism/*pharmacology
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 0923-1811(94)90040-X [pii]
AID - 10.1016/0923-1811(94)90040-x [doi]
PST - ppublish
SO  - J Dermatol Sci. 1994 Jul;7 Suppl:S98-103. doi: 10.1016/0923-1811(94)90040-x.