PMID- 8001457
OWN - NLM
STAT- MEDLINE
DCOM- 19950125
LR  - 20041117
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 17
IP  - 1
DP  - 1994 Sep 1
TI  - Flow-cytometric quantification in human gliomas of alpha satellite DNA sequences 
      specific for chromosome 7 using fluorescence in situ hybridization.
PG  - 26-32
AB  - Cell suspensions prepared from freshly frozen tissue specimens were used to 
      examine aberrations in the number of chromosome 7 signals in 10 human gliomas. 
      Nuclear DNA was hybridized in vitro with an alpha satellite DNA probe specific 
      for the centromeric regions of chromosome 7, using fluorescence in situ 
      hybridization (FISH). The intensity of the fluorescence signal from the 
      hybridized probe was measured, together with the nuclear DNA content, by flow 
      cytometry. The mean probe fluorescence of all nuclei was compared to the mean 
      copy number per nucleus found with microscopic scoring. Moreover, the mean probe 
      fluorescence ratio of DNA aneuploid nuclei relative to DNA diploid nuclei 
      (FISHa/FISHd) was calculated to determine how the numerical aberration of 
      chromosome 7 signals contributes to the DNA ploidy of the sample. The results 
      from the flow-cytometric analysis and from microscopic evaluation were 
      compatible. One of four tumors with DNA diploidy had a higher average intensity 
      of FISH signal and a broader coefficient of variation in FISH signal than normal 
      brain tissue; this was shown to be due to the gain of chromosome 7 signals. 
      Although FISHa/FISHd correlated with DNA indices (P < 0.01), there were some 
      disparities, probably due to other complex genotypic associations involving 
      several gains or losses of chromosomes. Thus gain of chromosome 7 in gliomas is 
      related to both DNA ploidy change and chromosome specific gain. It is concluded 
      that flow-cytometric quantification of FISH is useful in investigating numerical 
      aberrations of chromosomes and nuclear DNA content simultaneously.
FAU - Kwak, T
AU  - Kwak T
AD  - Department of Neurosurgery, Yamaguchi University School of Medicine, Japan.
FAU - Nishizaki, T
AU  - Nishizaki T
FAU - Ito, H
AU  - Ito H
FAU - Kimura, Y
AU  - Kimura Y
FAU - Murakami, T
AU  - Murakami T
FAU - Sasaki, K
AU  - Sasaki K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA, Satellite)
SB  - IM
MH  - *Aneuploidy
MH  - Astrocytoma/*chemistry/pathology
MH  - Brain Neoplasms/*chemistry/pathology
MH  - Cell Nucleus
MH  - *Chromosomes, Human, Pair 7
MH  - DNA, Neoplasm/*analysis
MH  - DNA, Satellite/*analysis
MH  - *Flow Cytometry
MH  - Frozen Sections
MH  - Glioblastoma/*chemistry/pathology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 10.1002/cyto.990170104 [doi]
PST - ppublish
SO  - Cytometry. 1994 Sep 1;17(1):26-32. doi: 10.1002/cyto.990170104.