PMID- 8002495
OWN - NLM
STAT- MEDLINE
DCOM- 19950120
LR  - 20171213
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 77
IP  - 2
DP  - 1994 Aug
TI  - Impaired plasma FFA oxidation imposed by extreme CHO deficiency in contracting 
      rat skeletal muscle.
PG  - 517-25
AB  - The extent to which carbohydrate (CHO) availability affects free fatty acid (FFA) 
      metabolism in contracting skeletal muscle is not well characterized. To study 
      this question, rats were depleted of glycogen by swimming exercise and lard 
      feeding 24 h before perfusion of their isolated hindquarters. After 20 min of 
      preperfusion with a medium containing no glucose, palmitate (600 or 2,000 
      microM), and [1-14C]palmitate, flow was restricted to one hindlimb, which was 
      electrically stimulated for 2 min to further deplete muscles of glycogen. After 2 
      min of recovery, glucose was added to the perfusate at final concentrations of 0, 
      6, or 20 mM, and after another 3 min muscles were stimulated for 30 min. At 6 and 
      2,000 microM palmitate, glucose uptake after 30 min of stimulation averaged 23.5 
      +/- 9.3 and 45.9 +/- 10.6 mumol.g-1.h-1 with 6 and 20 mM glucose, respectively. 
      At 6 and 2,000 microM palmitate, palmitate uptake was lower (30-37%, P < 0.05) 
      with 0 than with 6 or 20 mM glucose. At 600 microM palmitate, percent palmitate 
      oxidation was higher (27%, P < 0.05) with 0 than with 6 or 20 mM glucose, 
      resulting in similar total palmitate oxidation with the three glucose 
      concentrations (0.28 +/- 0.01 mumol.g-1.h-1). At 2,000 microM palmitate, percent 
      palmitate oxidation was not significantly different among glucose concentrations, 
      resulting in a significantly lower rate of palmitate oxidation with 0 (0.62 +/- 
      0.18 mumol.g-1.h-1) than with 6 or 20 mM glucose (0.77 +/- 0.25 and 0.78 +/- 0.20 
      mumol.g-1.h-1, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Turcotte, L P
AU  - Turcotte LP
AD  - Department of Exercise Sciences, University of Southern California, Los Angeles 
      90089-0652.
FAU - Hespel, P J
AU  - Hespel PJ
FAU - Graham, T E
AU  - Graham TE
FAU - Richter, E A
AU  - Richter EA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Carbohydrates)
RN  - 0 (Fatty Acids, Nonesterified)
RN  - 0 (Lactates)
RN  - 0 (Palmitates)
RN  - 0 (Phosphates)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Carbohydrates/*deficiency
MH  - Citric Acid Cycle/physiology
MH  - Erythrocytes/metabolism
MH  - Fatty Acids, Nonesterified/blood/*metabolism
MH  - Glucose/metabolism
MH  - Hindlimb/physiology
MH  - In Vitro Techniques
MH  - Lactates/blood
MH  - Male
MH  - Muscle Contraction/physiology
MH  - Muscle, Skeletal/*metabolism/physiology
MH  - Oxidation-Reduction
MH  - Oxygen Consumption/physiology
MH  - Palmitates/metabolism
MH  - Phosphates/metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 10.1152/jappl.1994.77.2.517 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 1994 Aug;77(2):517-25. doi: 10.1152/jappl.1994.77.2.517.