PMID- 8003001
OWN - NLM
STAT- MEDLINE
DCOM- 19940714
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 201
IP  - 2
DP  - 1994 Jun 15
TI  - Growth inhibition of SV40-transformed human keratinocytes by TGF-beta s is not 
      linked to dephosphorylation of the Rb gene product.
PG  - 673-81
AB  - We found that transforming growth factor beta 1 (TGF-beta 1) and TGF-beta 2 
      inhibited the growth of normal human keratinocytes and their SV40-transformed 
      counterpart in a dose dependent manner. Both normal and SV-40 transformed 
      keratinocytes accumulated in G1 when treated with TGF-beta s. The 
      hyperphosphorylated form of Rb gene product (RB) was reduced by TGF-beta s in 
      normal keratinocytes. In contrast, phosphorylation of RB was not essentially 
      affected in SV-40 transformed cells. This uncoupling of growth kinetics and 
      phosphorylation states of RB in SV-40 transformed keratinocytes suggests that RB 
      is not the primary target of action for TGF-beta s and that additional factors or 
      pathways may be involved in mechanisms of the growth inhibition.
FAU - Koike, M
AU  - Koike M
AD  - Department of Cancer Cell Research, University of Tokyo, Japan.
FAU - Ishino, K
AU  - Ishino K
FAU - Huh, N
AU  - Huh N
FAU - Kuroki, T
AU  - Kuroki T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Retinoblastoma Protein)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-49-2 (DNA)
SB  - IM
GS  - Rb
MH  - Blotting, Western
MH  - Cell Cycle/drug effects
MH  - Cell Division/drug effects
MH  - Cell Line, Transformed
MH  - Cells, Cultured
MH  - DNA/biosynthesis
MH  - Genes, Retinoblastoma
MH  - Humans
MH  - Keratinocytes/*cytology/drug effects/metabolism
MH  - Kinetics
MH  - Phosphorylation
MH  - Retinoblastoma Protein/isolation & purification/*metabolism
MH  - Simian virus 40/*genetics
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1994/06/15 00:00
MHDA- 1994/06/15 00:01
CRDT- 1994/06/15 00:00
PHST- 1994/06/15 00:00 [pubmed]
PHST- 1994/06/15 00:01 [medline]
PHST- 1994/06/15 00:00 [entrez]
AID - S0006-291X(84)71753-0 [pii]
AID - 10.1006/bbrc.1994.1753 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1994 Jun 15;201(2):673-81. doi: 
      10.1006/bbrc.1994.1753.