PMID- 8005590
OWN - NLM
STAT- MEDLINE
DCOM- 19940719
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 93
IP  - 6
DP  - 1994 Jun
TI  - Delineation of marker chromosomes by reverse chromosome painting using only a 
      small number of DOP-PCR amplified microdissected chromosomes.
PG  - 663-7
AB  - A new procedure for determining the chromosomal origin of marker chromosomes has 
      been carried out. The origin of marker chromosomes that were unidentifiable by 
      standard banding techniques could be verified by reverse chromosome painting. 
      This technique includes microdissection, followed by in vitro DNA amplification 
      and fluorescence in situ hybridization (FISH). A number of marker chromosomes 
      prepared from unbanded and from GTG-banded lymphocyte chromosomes were collected 
      with microneedles and transferred to a collection drop. The chromosomal material 
      was amplified by a degenerate oligonucleotide-primed polymerase chain reaction 
      (DOP-PCR). The resulting PCR products were labelled by nick-translation with 
      biotin-11-dUTP and used as probes for FISH. They were hybridized onto normal 
      metaphase spreads in order to determine the precise regional chromosomal origin 
      of the markers. Following this approach, we tested 2-14 marker chromosomes in 
      order to determine how many are necessary for reverse chromosome painting. As few 
      as two marker chromosomes provided sufficient material to paint the appropriate 
      chromosome of origin, regardless of whether the marker contained heterochromatic 
      or mainly euchromatic material. With this method, it was possible to identify two 
      marker chromosomes of a healthy proband [karyotype: 48,XY,+mar1,+mar2] and an 
      aberrant Y chromosome of a mentally retarded boy [karyotype: 46,X, der(Y)].
FAU - Viersbach, R
AU  - Viersbach R
AD  - Institut fur Humangenetik, Universitat Bonn, Germany.
FAU - Schwanitz, G
AU  - Schwanitz G
FAU - Nothen, M M
AU  - Nothen MM
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (DNA Primers)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Child, Preschool
MH  - Chromosome Banding
MH  - DNA Primers
MH  - *Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Karyotyping
MH  - Male
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1007/BF00201567 [doi]
PST - ppublish
SO  - Hum Genet. 1994 Jun;93(6):663-7. doi: 10.1007/BF00201567.