PMID- 8006586
OWN - NLM
STAT- MEDLINE
DCOM- 19940721
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 180
IP  - 1
DP  - 1994 Jul 1
TI  - Identification of a human cDNA encoding a functional high affinity lipoxin A4 
      receptor.
PG  - 253-60
AB  - Lipoxin A4 (LXA4) triggers selective responses with human neutrophils that are 
      pertussis toxin sensitive and binds to high affinity receptors (Kd = 0.5 +/- 0.3 
      nM) that are modulated by stable analogues of guanosine 5'-triphosphate (GTP). 
      Here, we characterized [11,12-(3)]LXA4 specific binding with neutrophil granule 
      and plasma membranes, which each display high affinity binding sites (Kd = 0.7 
      +/- 0.1 nM) that were regulated by GTP gamma S. Since functional LXA4 receptors 
      are inducible in HL-60 cells, we tested orphan cDNAs encoding 7-transmembrane 
      region receptors cloned from these cells for their ability to bind and signal 
      with LXA4. Chinese hamster ovary (CHO) cells transfected with the orphan receptor 
      cDNA (pINF114) displayed specific 3H-LXA4 high affinity binding (1.7 nM). When 
      displacement of LXA4 binding with pINF114-transfected CHO cells was tested with 
      other eicosanoids, including LXB4, leukotriene D4 (LTD4), LTB4, or prostaglandin 
      E2, only LTD4 competed with LXA4, giving a Ki of 80 nM. In transfected CHO cells, 
      LXA4 also stimulated GTPase activity and provoked the release of esterified 
      arachidonate, which proved to be pertussis toxin sensitive. These results 
      indicate that pINF114 cDNA encodes a 7-transmembrane region-containing protein 
      that displays high affinity for 3H-LXA4 and transmits LXA4-induced signals. 
      Together, they suggest that the encoded protein is a candidate for a LXA4 
      receptor in myeloid cells.
FAU - Fiore, S
AU  - Fiore S
AD  - Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 
      02115.
FAU - Maddox, J F
AU  - Maddox JF
FAU - Perez, H D
AU  - Perez HD
FAU - Serhan, C N
AU  - Serhan CN
LA  - eng
GR  - GM-38765/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (DNA, Complementary)
RN  - 0 (Hydroxyeicosatetraenoic Acids)
RN  - 0 (Lipoxins)
RN  - 0 (Receptors, Eicosanoid)
RN  - 0 (lipoxin A4)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arachidonic Acid/metabolism
MH  - CHO Cells
MH  - Cricetinae
MH  - DNA, Complementary/*analysis
MH  - Humans
MH  - Hydroxyeicosatetraenoic Acids/*metabolism/pharmacology
MH  - *Lipoxins
MH  - Molecular Sequence Data
MH  - N-Formylmethionine Leucyl-Phenylalanine/metabolism/pharmacology
MH  - Neutrophils/metabolism
MH  - Receptors, Eicosanoid/*genetics
PMC - PMC2191537
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
PMCR- 1995/01/01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
PHST- 1995/01/01 00:00 [pmc-release]
AID - 94275379 [pii]
AID - 10.1084/jem.180.1.253 [doi]
PST - ppublish
SO  - J Exp Med. 1994 Jul 1;180(1):253-60. doi: 10.1084/jem.180.1.253.