PMID- 8012362
OWN - NLM
STAT- MEDLINE
DCOM- 19940728
LR  - 20190512
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 3
IP  - 3
DP  - 1994 Mar
TI  - Integration of physical, genetic and cytogenetic maps of human chromosome 7: 
      isolation and analysis of yeast artificial chromosome clones for 117 mapped 
      genetic markers.
PG  - 489-501
AB  - An important goal for the human genome project is to assemble fully integrated 
      physical, genetic and cytogenetic maps for each human chromosome. Towards that 
      end, we have isolated yeast artificial chromosome (YAC) clones containing 117 of 
      the 119 genetic markers that constitute a recently constructed, detailed genetic 
      map of human chromosome 7. Analysis of these clones reveals numerous examples 
      where adjacent genetic markers have been physically connected, either in 
      individual YACs or in multi-YAC contigs. At present, the 117 genetic markers are 
      contained in fewer than 80 YAC contigs, with most of these contigs uniquely 
      ordered relative to one another based on the genetic map positions of the 
      corresponding markers. These YACs and YAC contigs are estimated to contain 
      approximately 60-85% of the DNA from human chromosome 7. YACs representing 36 
      genetic markers were mapped by fluorescence in situ hybridization (FISH) to 
      metaphase chromosomes, allowing assignment of these genetic markers to 
      cytogenetic bands along chromosome 7 and placement of the centromere within the 
      genetic map. Together, these studies provide genetically and cytogenetically 
      anchored YAC clones covering the majority of chromosome 7 that will be useful 
      both for the positional cloning of genes and as a framework for assembling a 
      complete YAC-based physical map of the chromosome.
FAU - Green, E D
AU  - Green ED
AD  - Department of Genetics, Washington University School of Medicine, St. Louis, MO 
      63110.
FAU - Idol, J R
AU  - Idol JR
FAU - Mohr-Tidwell, R M
AU  - Mohr-Tidwell RM
FAU - Braden, V V
AU  - Braden VV
FAU - Peluso, D C
AU  - Peluso DC
FAU - Fulton, R S
AU  - Fulton RS
FAU - Massa, H F
AU  - Massa HF
FAU - Magness, C L
AU  - Magness CL
FAU - Wilson, A M
AU  - Wilson AM
FAU - Kimura, J
AU  - Kimura J
AU  - et al.
LA  - eng
GR  - HG00256/HG/NHGRI NIH HHS/United States
GR  - HG00774-02/HG/NHGRI NIH HHS/United States
GR  - P50-HG00201/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 7
MH  - Cloning, Molecular
MH  - *Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
AID - 10.1093/hmg/3.3.489 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1994 Mar;3(3):489-501. doi: 10.1093/hmg/3.3.489.