PMID- 8013077
OWN - NLM
STAT- MEDLINE
DCOM- 19940726
LR  - 20220311
IS  - 0009-7330 (Print)
IS  - 0009-7330 (Linking)
VI  - 75
IP  - 1
DP  - 1994 Jul
TI  - Cytokine-stimulated human vascular smooth muscle cells synthesize a complement of 
      enzymes required for extracellular matrix digestion.
PG  - 181-9
AB  - Vascular matrix remodeling occurs during development, growth, and several 
      pathological conditions that affect blood vessels. We investigated the capacity 
      of human smooth muscle cells (SMCs) to express matrix metalloproteinases (MMPs), 
      enzymes that selectively digest components of the extracellular matrix (ECM), in 
      the basal state or after stimulation with certain cytokines implicated in 
      vascular homeostasis and pathology. Enzymatic activity associated with various 
      proteins secreted in the culture media was detected by gelatin or casein sodium 
      dodecyl sulfate-polyacrylamide gel electrophoresis zymography. Proteins were 
      identified by immunoprecipitation and mRNA by Northern blotting. SMCs 
      constitutively secreted a 72-kD gelatinase and the tissue inhibitors of MMPs 
      (TIMPs) types 1 and 2. SMCs stimulated with interleukin-1 or tumor necrosis 
      factor-alpha synthesized de novo 92-kD gelatinase, interstitial collagenase, and 
      stromelysin. Several lines of evidence suggest that when stimulated by cytokines, 
      SMCs produce activated forms of MMPs. Together, the constitutive and the 
      cytokine-induced enzymes can digest all the major components of the vascular ECM. 
      Moreover, since these mediators augment the production of MMPs without 
      appreciably affecting the synthesis of TIMPs, locally secreted cytokines may tip 
      the regional balance of MMP activity in favor of vascular matrix degradation.
FAU - Galis, Z S
AU  - Galis ZS
AD  - Brigham and Women's Hospital, Boston, MA 02115.
FAU - Muszynski, M
AU  - Muszynski M
FAU - Sukhova, G K
AU  - Sukhova GK
FAU - Simon-Morrissey, E
AU  - Simon-Morrissey E
FAU - Unemori, E N
AU  - Unemori EN
FAU - Lark, M W
AU  - Lark MW
FAU - Amento, E
AU  - Amento E
FAU - Libby, P
AU  - Libby P
LA  - eng
GR  - HL-34636-10/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Circ Res
JT  - Circulation research
JID - 0047103
RN  - 0 (Cytokines)
RN  - 0 (Glycoproteins)
RN  - 0 (Interleukin-1)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Collagenases)
RN  - EC 3.4.24.- (Gelatinases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Cells, Cultured
MH  - Collagenases/biosynthesis
MH  - Cytokines/*pharmacology
MH  - Enzyme Activation
MH  - Extracellular Matrix/*metabolism
MH  - Extracellular Space/metabolism
MH  - Gelatinases/metabolism
MH  - Glycoproteins/biosynthesis
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Intracellular Membranes/metabolism
MH  - Matrix Metalloproteinase 3
MH  - Matrix Metalloproteinase Inhibitors
MH  - Metalloendopeptidases/antagonists & inhibitors/biosynthesis/metabolism
MH  - Muscle, Smooth, Vascular/cytology/*metabolism
MH  - Tissue Inhibitor of Metalloproteinases
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1161/01.res.75.1.181 [doi]
PST - ppublish
SO  - Circ Res. 1994 Jul;75(1):181-9. doi: 10.1161/01.res.75.1.181.