PMID- 8017858
OWN - NLM
STAT- MEDLINE
DCOM- 19940725
LR  - 20071115
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 14
IP  - 2A
DP  - 1994 Mar-Apr
TI  - Detection of unexpected clones of monosomy 7 in childhood acute lymphoblastic 
      leukemia using fluorescence in situ hybridization.
PG  - 545-8
AB  - The feasibility of a fluorescence in situ hybridization (FISH) technique for the 
      detection of leukemic clones with masked chromosomal aberration in interphase 
      nuclei was tested in childhood acute lymphoblastic leukemia (ALL). Twenty-one 
      cases of ALL previously studied by classical metaphase cytogenetics were 
      retrospectively analysed using a centromere-specific chromosome 7 probe. Five 
      cases with karyotypic abnormalities of chromosome 7 (2 with trisomy 7, 2 with 
      monosomy 7 and 1 with trisomy & tetrasomy 7) showed a correlation with FISH 
      results, whereas in five other cases monosomy 7 was found in 12-43% of cells only 
      by FISH. The unexpected detection of monosomy 7 in these latter ALL patients 
      suggests that either these clones are quiescent or unable to enter mitosis in 
      vitro. This suggests that FISH and metaphase cytogenetics must be combined 
      whenever possible to obtain comprehensive karyotypic information.
FAU - Kadam, P
AU  - Kadam P
AD  - Rush-Presbyterian St.-Luke's Medical Center, Chicago, IL 60612.
FAU - Masterson, M
AU  - Masterson M
FAU - Soukup, S
AU  - Soukup S
FAU - Moore, C
AU  - Moore C
FAU - Raza, A
AU  - Raza A
FAU - Lampkin, B C
AU  - Lampkin BC
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
SB  - IM
MH  - Bone Marrow/pathology
MH  - Burkitt Lymphoma/genetics/pathology
MH  - Child
MH  - *Chromosomes, Human, Pair 7
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Leukemia-Lymphoma, Adult T-Cell/genetics/pathology
MH  - *Monosomy
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics/pathology
MH  - Preleukemia/*genetics/pathology
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1994 Mar-Apr;14(2A):545-8.