PMID- 8019469
OWN - NLM
STAT- MEDLINE
DCOM- 19940729
LR  - 20061115
IS  - 0268-3369 (Print)
IS  - 0268-3369 (Linking)
VI  - 13
IP  - 4
DP  - 1994 Apr
TI  - Detection of chimerism in subpopulations of cells by fluorescence in situ 
      hybridization and immunofluorescent staining of cell surface antigens.
PG  - 441-7
AB  - Fluorescence in situ hybridization (FISH) enables the visualization of the X and 
      Y chromosomes in nuclei of interphase cells using probes which recognize 
      repetitive sequences on the X and Y chromosomes. The process of cell preparation, 
      hybridization and visualization of the probes within the nuclei lasts between 16 
      and 24 h. Because of these features the method is ideal for monitoring 
      engraftment after sex-mismatched bone marrow transplant (BMT). So far, the FISH 
      technique has been applied to assess the degree of chimerism in either 
      unseparated peripheral blood cells or unseparated bone marrow cells. However, for 
      early detection of rejection or relapse, the degree of chimerism in 
      subpopulations of cells is probably more informative. A new FISH protocol was 
      developed with the aim of simultaneously visualizing the surface antigens by 
      fluorescent antibodies and X or Y chromosomes with fluorescent probes. After 
      development of the new FISH protocol, it was applied to patient samples after 
      sex-mismatched BMT. The degree of chimerism was assessed comparing the results of 
      the new FISH protocol with a 'standard' FISH protocol which is employed both in 
      our and other laboratories to detect chromosomes in interphase nuclei. Both 
      methods gave similar results. The new FISH protocol was applicable for the 
      detection of Y and X chromosomes. The clinical relevance of this new FISH 
      protocol is illustrated by the results obtained on peripheral blood samples of 
      five BMT patients who were mixed chimeras after sex-mismatched BMT. A clear 
      distinction between engraftment of T and B cells was noted. In some patients the 
      T cells were of donor origin while the B cells were of host origin.
FAU - Gerritsen, W R
AU  - Gerritsen WR
AD  - Department of Pediatrics, Memorial Sloan-Kettering Cancer Center, New York.
FAU - Jagiello, C A
AU  - Jagiello CA
FAU - Bourhis, J H
AU  - Bourhis JH
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Bone Marrow Transplant
JT  - Bone marrow transplantation
JID - 8702459
RN  - 0 (Antigens, Surface)
SB  - IM
MH  - Adult
MH  - Antigens, Surface/*analysis/genetics
MH  - Blood Cells/*chemistry
MH  - Bone Marrow Transplantation/*pathology
MH  - Child
MH  - Child, Preschool
MH  - Chimera
MH  - Female
MH  - *Fluorescent Antibody Technique
MH  - *Graft Survival
MH  - Humans
MH  - Immunologic Deficiency Syndromes/therapy
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia/therapy
MH  - Male
MH  - *X Chromosome
MH  - *Y Chromosome
MH  - beta-Thalassemia/therapy
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
PST - ppublish
SO  - Bone Marrow Transplant. 1994 Apr;13(4):441-7.