PMID- 8019967
OWN - NLM
STAT- MEDLINE
DCOM- 19940801
LR  - 20220331
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 74
IP  - 2
DP  - 1994 Jun
TI  - A new B-cell line showing a complex translocation (8;14;18) and BCL2 
      rearrangement.
PG  - 87-94
AB  - A cell line named ROS-50 (Rotterdam suspension cell line no. 50) has been 
      established from peripheral blood of a 69-year-old male with acute lymphoblastic 
      leukemia (FAB type L3). Among the aberrations, cytogenetic analysis showed the 
      presence of 14q+, 18q-, and two 8q- marker chromosomes. With fluorescence in situ 
      hybridization (FISH) we characterized the chromosomal translocations, t(8;14) and 
      t(14;18), in which the same chromosome 14 is involved. PCR analysis demonstrated 
      the presence of on IGH-BCL2 rearrangement with a breakpoint in the minor cluster 
      region (mcr) confirming the t(14;18) characteristic for follicular lymphoma. 
      Additional studies showed high expression of BCL2 protein, an early B-cell 
      immunophenotype, and an unusual pattern of IGH gene rearrangement.
FAU - van Ooteghem, R B
AU  - van Ooteghem RB
AD  - Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The 
      Netherlands.
FAU - Smit, E M
AU  - Smit EM
FAU - Beishuizen, A
AU  - Beishuizen A
FAU - Lambrechts, A C
AU  - Lambrechts AC
FAU - vd Blij-Philipsen, M
AU  - vd Blij-Philipsen M
FAU - Smilde, T J
AU  - Smilde TJ
FAU - Hagemeijer, A
AU  - Hagemeijer A
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Aged
MH  - Base Sequence
MH  - *Cell Line
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 14
MH  - Chromosomes, Human, Pair 18
MH  - Chromosomes, Human, Pair 8
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Molecular Sequence Data
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proto-Oncogene Proteins c-bcl-2
MH  - *Translocation, Genetic
RF  - 31
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 0165-4608(94)90003-5 [pii]
AID - 10.1016/0165-4608(94)90003-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1994 Jun;74(2):87-94. doi: 10.1016/0165-4608(94)90003-5.