PMID- 8020937
OWN - NLM
STAT- MEDLINE
DCOM- 19940729
LR  - 20190904
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 20
IP  - 1
DP  - 1994 Mar 1
TI  - Localization of the squalene synthase gene (FDFT1) to human chromosome 
      8p22-p23.1.
PG  - 116-8
AB  - Recently, we reported the isolation of a cDNA encoding the human enzyme squalene 
      synthase, the first step of sterol biosynthesis uniquely committed to synthesis 
      of cholesterol (6). As such, it is likely that this enzyme occupies a critical 
      regulatory position in the synthesis of cholesterol. As part of continuing 
      studies of the role of this gene in cellular metabolism, we undertook the mapping 
      of this gene on the human chromosomes. To localize the gene, we have first 
      isolated a yeast artificial chromosome (YAC) containing the squalene synthase 
      gene. We then used fluorescence in situ hybridization (FISH) with yeast DNA 
      containing the YAC to localize the gene to chromosome 8. Assignment to human 
      chromosome 8 was confirmed by polymerase chain reaction analysis of a somatic 
      cell hybrid containing human chromosome 8. Use of a somatic cell hybrid regional 
      mapping panel dividing chromosome 8 into several fragments localized the gene to 
      8p21-pter. Fractional length analysis of the FISH mapping placed the signal 
      generated with this YAC at 8p22-p23.1.
FAU - Shechter, I
AU  - Shechter I
AD  - Eleanor Roosevelt Institute, Denver, Colorado 80206.
FAU - Conrad, D G
AU  - Conrad DG
FAU - Hart, I
AU  - Hart I
FAU - Berger, R C
AU  - Berger RC
FAU - McKenzie, T L
AU  - McKenzie TL
FAU - Bleskan, J
AU  - Bleskan J
FAU - Patterson, D
AU  - Patterson D
LA  - eng
GR  - AG00029/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (DNA Primers)
RN  - EC 2.5.1.21 (Farnesyl-Diphosphate Farnesyltransferase)
SB  - IM
GS  - FDFT1
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 8/ultrastructure
MH  - DNA Primers/genetics
MH  - Farnesyl-Diphosphate Farnesyltransferase/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
AID - S0888754384711359 [pii]
AID - 10.1006/geno.1994.1135 [doi]
PST - ppublish
SO  - Genomics. 1994 Mar 1;20(1):116-8. doi: 10.1006/geno.1994.1135.