PMID- 8020959
OWN - NLM
STAT- MEDLINE
DCOM- 19940729
LR  - 20201209
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 20
IP  - 1
DP  - 1994 Mar 1
TI  - Identification of YAC and cosmid clones encompassing the ZFX-POLA region using 
      irradiation hybrid cell lines.
PG  - 75-83
AB  - The human Xp21.3-p22.1 region is poorly mapped relative to other X chromosome 
      regions. To target cosmid and YAC clones specifically from Xp21.3-p22.1 for rapid 
      contig construction, a hybridization-based screening approach using irradiation 
      hybrids has been used. Alu-PCR products generated from hybrid lines containing 
      small overlapping fragments from Xp21-p22 were hybridized to an X chromosome 
      cosmid library, and cosmids predicted by their hybridization pattern to map to 
      the region of interest were analyzed by fluorescence in situ hybridization 
      (FISH). Hybridization of the cosmids in pools to gridded YAC libraries identified 
      15 YACs, which were verified and tested for chimerism by FISH. Cosmid content 
      analysis of the YACs defined two contigs, one with 12 YACs covering about 1.5 Mb 
      and one with 3 YACs. Five YACs from the 12-YAC cluster had been previously 
      recognized by DNA polymerase alpha (POLA). ZFX identified a single YAC; hence, 
      the physical linkage of ZFX and POLA was demonstrated within the contig. Four 
      YACs had been isolated previously with ZFX and these extend the contig to 2 Mb. 
      Restriction mapping of several YACs demonstrates that ZFX and POLA are about 700 
      kb apart, a distance similar to that reported in the mouse between Zfx and Pola. 
      The order of these two loci and two additional loci identified by homologous 
      mouse linking clones was found to be conserved between human and mouse: 
      tel-ZFX-DXCrc57-DXCrc140-POLA-cen. We have shown that YAC contigs can be rapidly 
      constructed from targeted regions without the need for time-consuming YAC end 
      rescue and chromosomal walking.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Francis, F
AU  - Francis F
AD  - Genome Analysis Laboratory, Imperial Cancer Research Fund, London, United 
      Kingdom.
FAU - Benham, F
AU  - Benham F
FAU - See, C G
AU  - See CG
FAU - Fox, M
AU  - Fox M
FAU - Ishikawa-Brush, Y
AU  - Ishikawa-Brush Y
FAU - Monaco, A P
AU  - Monaco AP
FAU - Weiss, B
AU  - Weiss B
FAU - Rappold, G
AU  - Rappold G
FAU - Hamvas, R M
AU  - Hamvas RM
FAU - Lehrach, H
AU  - Lehrach H
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Genetic Markers)
RN  - EC 2.7.7.7 (DNA Polymerase II)
SB  - IM
GS  - POLA
GS  - ZFX
MH  - Animals
MH  - Chromosome Mapping
MH  - *Chromosomes, Artificial, Yeast
MH  - Cloning, Molecular
MH  - Cosmids/genetics
MH  - DNA Polymerase II/genetics
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Gene Library
MH  - Genetic Linkage
MH  - Genetic Markers
MH  - Humans
MH  - Hybrid Cells/radiation effects
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Species Specificity
MH  - *X Chromosome
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
AID - S0888-7543(84)71129-3 [pii]
AID - 10.1006/geno.1994.1129 [doi]
PST - ppublish
SO  - Genomics. 1994 Mar 1;20(1):75-83. doi: 10.1006/geno.1994.1129.