PMID- 8020967
OWN - NLM
STAT- MEDLINE
DCOM- 19940804
LR  - 20061115
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 20
IP  - 2
DP  - 1994 Mar 15
TI  - Physical mapping of chromosome 12q breakpoints in lipoma, pleomorphic salivary 
      gland adenoma, uterine leiomyoma, and myxoid liposarcoma.
PG  - 210-22
AB  - We report here the physical mapping of recurrent chromosome 12q13-q15 breakpoints 
      in cell lines derived from primary myxoid liposarcoma, lipoma, uterine leiomyoma, 
      and pleomorphic adenoma of the salivary glands. In fluorescence in situ 
      hybridization (FISH) experiments, we first mapped the position of the chromosome 
      12 translocation breakpoint in uterine leiomyoma cell line LM-30.1/SV40 relative 
      to loci COL2A1, D12S4, D12S17, D12S6, D12S6, D12S19, D12S8, and D12S7. It mapped 
      between linkage probes CRI-C86 (D12S19) and p7G11 (D12S8). We then isolated YAC 
      clones using CRI-C86- and p7G11-derived sequence-tagged sites, constructed 
      corresponding YAC contigs of 310 and 800 kb, respectively, and established 
      long-range physical maps of these. Cosmid clones LLNL12NCO1-98C10 and 
      LLNL12NCO1-113D12 were isolated using STSs within the CRI-C86- and the 
      p7G11-derived YAC contigs, respectively, and a mixture of them was used to 
      routinely study the various tumor cell lines by FISH analysis. The chromosome 12 
      breakpoints of all tumor cell lines tested mapped between cosmids 
      LLNL12NCO1-98C10 and LLNL12NCO1-113D12. None of the breakpoints appeared to map 
      within any of the isolated YAC clones. Furthermore, FISH analysis using cosmid 
      LLNL12-NCO1-144G3, which maps at the CHOP locus, revealed that the chromosome 12 
      breakpoints in all cell lines of the three benign solid tumors that were tested 
      were located distal to the chromosome 12 translocation breakpoint with the CHOP 
      gene in myxoid liposarcoma cells with t(12;16). In conclusion, our studies seem 
      to indicate that the chromosome 12 breakpoints of myxoid liposarcoma, lipoma, 
      uterine leiomyoma, and pleomorphic adenoma of the salivary glands are all 
      clustered within the 7-cM interval between D12S19 and D12S8, with those of the 
      benign solid tumors distal to CHOP. Finally, the MYF5 gene mapped telomeric to 
      LLNL12NCO1-113D12, and the MIP gene mapped centromeric to the chromosome 12 
      translocation breakpoint in myxoid liposarcoma cells.
FAU - Schoenmakers, E F
AU  - Schoenmakers EF
AD  - Center for Human Genetics, University of Leuven, Belgium.
FAU - Kools, P F
AU  - Kools PF
FAU - Mols, R
AU  - Mols R
FAU - Kazmierczak, B
AU  - Kazmierczak B
FAU - Bartnitzke, S
AU  - Bartnitzke S
FAU - Bullerdiek, J
AU  - Bullerdiek J
FAU - Dal Cin, P
AU  - Dal Cin P
FAU - De Jong, P J
AU  - De Jong PJ
FAU - Van den Berghe, H
AU  - Van den Berghe H
FAU - Van de Ven, W J
AU  - Van de Ven WJ
LA  - eng
SI  - GENBANK/U26689
SI  - GENBANK/U27130
SI  - GENBANK/U27131
SI  - GENBANK/U27132
SI  - GENBANK/U27133
SI  - GENBANK/U27134
SI  - GENBANK/U27135
SI  - GENBANK/U27136
SI  - GENBANK/U27137
SI  - GENBANK/U27138
SI  - GENBANK/U27139
SI  - GENBANK/U27140
SI  - GENBANK/U27141
SI  - GENBANK/U27142
SI  - GENBANK/U27145
SI  - GENBANK/U27146
SI  - GENBANK/U27147
SI  - GENBANK/U27148
SI  - GENBANK/U27149
SI  - GENBANK/U27150
SI  - GENBANK/U27151
SI  - GENBANK/U27152
SI  - GENBANK/U27153
SI  - GENBANK/U27154
SI  - GENBANK/U27155
SI  - GENBANK/U27156
SI  - GENBANK/U27157
SI  - GENBANK/U27158
SI  - GENBANK/U27159
SI  - GENBANK/U27160
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adenoma/genetics
MH  - Base Sequence
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 12
MH  - Cloning, Molecular
MH  - DNA
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leiomyoma/*genetics
MH  - Lipoma/*genetics
MH  - Liposarcoma, Myxoid/*genetics
MH  - Molecular Sequence Data
MH  - Restriction Mapping
MH  - Salivary Gland Neoplasms/*genetics
MH  - Tumor Cells, Cultured
MH  - Uterine Neoplasms/*genetics
EDAT- 1994/03/15 00:00
MHDA- 1994/03/15 00:01
CRDT- 1994/03/15 00:00
PHST- 1994/03/15 00:00 [pubmed]
PHST- 1994/03/15 00:01 [medline]
PHST- 1994/03/15 00:00 [entrez]
AID - S0888-7543(84)71155-4 [pii]
AID - 10.1006/geno.1994.1155 [doi]
PST - ppublish
SO  - Genomics. 1994 Mar 15;20(2):210-22. doi: 10.1006/geno.1994.1155.