PMID- 8021610
OWN - NLM
STAT- MEDLINE
DCOM- 19940804
LR  - 20081121
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 75 ( Pt 7)
DP  - 1994 Jul
TI  - Molecular cloning and nucleotide sequencing of the 3'-terminal genomic RNA of the 
      porcine reproductive and respiratory syndrome virus.
PG  - 1795-801
AB  - The genomic RNA of a porcine reproductive and respiratory syndrome virus (PRRSV) 
      isolate from the U.S.A., VR 2385 (ATCC), was copied into cDNA after priming with 
      oligo(dT) and cloned into phage lambda. The cDNA clones representing the 
      3'-terminal genomic RNA of the virus were isolated and sequenced. The genome is a 
      positive-stranded, polyadenylated RNA with an estimated size of 15 kb. Analysis 
      of the resulting sequence identified three complete open reading frames (ORFs) 
      with the potential to encode polypeptides with predicted M(r)s of 22.2K (ORF 5), 
      19.1K (ORF 6) and 13.6K (ORF 7). ORF 7, which is closest to the 3' end, is 
      predicted to encode a highly basic nucleocapsid protein displaying 58% amino acid 
      identity to the corresponding protein of the Lelystad virus (LV), a European 
      PRRSV isolate. ORFs 6 and 5, preceding ORF 7, are each predicted to encode 
      proteins containing several hydrophobic domains that are thought to be 
      membrane-associated. The VR 2385 ORF 6 protein is the most conserved structural 
      protein. It has 78% amino acid identity to the equivalent LV protein, and ORF 5 
      shares only 54% of its amino acid sequence. Northern blot analysis revealed a 
      3'-coterminal nested set of six subgenomic RNAs in VR 2385 virus-infected CRL 
      11171 cells. Our results indicate that VR 2385, like LV, is a member of the newly 
      proposed arterivirus group. However, the striking genetic variation and the 
      difference in pathogenicity between LV and VR 2385 suggest that the viruses 
      causing PRRS in the U.S.A. and Europe are highly variable and they may represent 
      different genotypes.
FAU - Meng, X J
AU  - Meng XJ
AD  - Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State 
      University, Ames 50011.
FAU - Paul, P S
AU  - Paul PS
FAU - Halbur, P G
AU  - Halbur PG
LA  - eng
SI  - GENBANK/U03040
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - Genetic Variation/genetics
MH  - Lung/microbiology
MH  - Molecular Sequence Data
MH  - Open Reading Frames/genetics
MH  - RNA, Viral/biosynthesis
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Swine
MH  - Swine Diseases/*microbiology
MH  - Transcription, Genetic
MH  - United States
MH  - Virus Diseases/microbiology/*veterinary
MH  - Viruses, Unclassified/*genetics
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1099/0022-1317-75-7-1795 [doi]
PST - ppublish
SO  - J Gen Virol. 1994 Jul;75 ( Pt 7):1795-801. doi: 10.1099/0022-1317-75-7-1795.