PMID- 8021818
OWN - NLM
STAT- MEDLINE
DCOM- 19940804
LR  - 20200220
IS  - 0894-9255 (Print)
IS  - 0894-9255 (Linking)
VI  - 7
IP  - 8
DP  - 1994 Aug
TI  - Identification of low-level contamination of blood as basis for detection of 
      human immunodeficiency virus (HIV) DNA in anti-HIV-negative specimens.
PG  - 853-9
AB  - The significance of detection of human immunodeficiency virus (HIV) DNA by the 
      polymerase chain reaction (PCR) in seronegative or seroconverting (SC) subjects 
      remains controversial. In a previously reported study, we identified a case in 
      which a specimen collected 12 months before seroconversion (pre-SC) was found 
      repeatedly to be PCR positive in three experienced laboratories, while the 
      6-month pre-SC bleed was PCR-negative; PCR-based human leukocyte antigen 
      (HLA)-DQA and -DRB typing of serial peripheral blood mononuclear cell (PBMC) 
      samples from this case did not indicate a specimen mix-up or labeling error. To 
      further investigate this case, we used HIV env sequence and DNA heteroduplex 
      gel-shift analyses to characterize HIV quasispecies present in serial pre- and 
      post-SC specimens. HIV env sequences and gel-shift pattern analyses from the 
      12-month pre-SC versus post-SC samples indicated that markedly distinct 
      quasispecies were present, suggesting possible abortive infection followed by 
      reinfection and subsequent seroconversion. However, the HIV burden of this pre-SC 
      sample was very low (1 provirus/10(6) PBMCs), and the quasispecies was highly 
      heterogeneous, findings suggesting long-term rather than recent HIV infection. To 
      test the hypothesis that the index pre-SC sample was PCR positive owing to trace 
      blood contamination during initial processing, we analyzed the three seropositive 
      samples collected on the same date in 1985. One of these samples was highly 
      related to the index pre-SC sample by env sequence and gel-shift 
      methodologies.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Sabino, E C
AU  - Sabino EC
AD  - Irwin Memorial Blood Centers, San Francisco, CA 94118.
FAU - Delwart, E
AU  - Delwart E
FAU - Lee, T H
AU  - Lee TH
FAU - Mayer, A
AU  - Mayer A
FAU - Mullins, J I
AU  - Mullins JI
FAU - Busch, M P
AU  - Busch MP
LA  - eng
SI  - GENBANK/L20371
SI  - GENBANK/L20372
SI  - GENBANK/L20373
SI  - GENBANK/L20374
SI  - GENBANK/L20375
SI  - GENBANK/L20376
SI  - GENBANK/L20377
SI  - GENBANK/L20378
SI  - GENBANK/L20379
SI  - GENBANK/L20380
GR  - N01-A1-82515/PHS HHS/United States
GR  - R01-HL-48367-A101/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Acquir Immune Defic Syndr (1988)
JT  - Journal of acquired immune deficiency syndromes
JID - 8812597
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (Gene Products, env)
RN  - 0 (HIV Antibodies)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (HIV Envelope Protein gp160)
RN  - 0 (HIV envelope protein gp120 (305-321))
RN  - 0 (HLA-DR2 Antigen)
RN  - 0 (Peptide Fragments)
RN  - 0 (Protein Precursors)
SB  - IM
GS  - env
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cell Separation
MH  - Consensus Sequence
MH  - DNA Primers/chemistry
MH  - DNA, Viral/*blood/chemistry
MH  - Diagnostic Errors
MH  - Gene Products, env/genetics/immunology
MH  - HIV Antibodies/*blood
MH  - HIV Envelope Protein gp120/genetics
MH  - HIV Envelope Protein gp160
MH  - *HIV Seronegativity
MH  - HIV Seropositivity/*diagnosis
MH  - HIV-1/*genetics/immunology/isolation & purification
MH  - HLA-DR2 Antigen/genetics
MH  - Humans
MH  - Leukocytes, Mononuclear/microbiology
MH  - Molecular Sequence Data
MH  - Peptide Fragments/genetics
MH  - Polymerase Chain Reaction
MH  - Protein Precursors/immunology
MH  - Sequence Homology, Nucleic Acid
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
PST - ppublish
SO  - J Acquir Immune Defic Syndr (1988). 1994 Aug;7(8):853-9.