PMID- 8024701
OWN - NLM
STAT- MEDLINE
DCOM- 19940808
LR  - 20220330
IS  - 1044-5498 (Print)
IS  - 1044-5498 (Linking)
VI  - 13
IP  - 6
DP  - 1994 Jun
TI  - beta ig-h3: a transforming growth factor-beta-responsive gene encoding a secreted 
      protein that inhibits cell attachment in vitro and suppresses the growth of CHO 
      cells in nude mice.
PG  - 571-84
AB  - beta ig-h3 is a novel gene first discovered by differential screening of a cDNA 
      library made from A549 human lung adenocarcinoma cells treated with transforming 
      growth factor-beta 1 (TGF-beta 1). It encodes a 683-amino-acid protein containing 
      a secretory signal sequence and four homologous internal domains. Here we show 
      that treatment of several types of cells, including human melanoma cells, human 
      mammary epithelial cells, human keratinocytes, and human fibroblasts, with 
      TGF-beta resulted in a significant increase in beta ig-h3 RNA. A portion of the 
      beta ig-h3 coding sequence was expressed in bacteria, and antisera against the 
      bacterially produced protein was raised in rabbits. This antisera was used to 
      demonstrate that several cell lines secreted a 68-kD beta IG-H3 protein after 
      treatment with TGF-beta. Transfection of beta IG-H3 expression plasmids into 
      Chinese hamster ovary (CHO) cells led to a marked decrease in the ability of 
      these cells to form tumors in nude mice. The beta IG-H3 protein was purified from 
      media conditioned by recombinant CHO cells, characterized by immunoblotting and 
      protein sequencing and shown to function in an anti-adhesion assay in that it 
      inhibited the attachment of A549, HeLa, and WI-38 cells to plastic in serum-free 
      media. Sequencing of cDNA clones encoding murine beta ig-H3 indicated 90.6% 
      conservation at the amino acid level between the murine and human proteins. 
      Finally, the beta ig-h3 gene was localized to human chromosome 5q31, a region 
      frequently deleted in preleukemic myelodysplasia and leukemia. The corresponding 
      mouse beta ig-h3 gene was mapped to mouse chromosome 13 region B to C1, which 
      confirms a region of conservation on human chromosome 5 and mouse chromosome 13. 
      We suggest that this protein be named p68 beta ig-h3.
FAU - Skonier, J
AU  - Skonier J
AD  - Bristol-Myers Squibb, Pharmaceutical Research Institute Seattle, WA 98121.
FAU - Bennett, K
AU  - Bennett K
FAU - Rothwell, V
AU  - Rothwell V
FAU - Kosowski, S
AU  - Kosowski S
FAU - Plowman, G
AU  - Plowman G
FAU - Wallace, P
AU  - Wallace P
FAU - Edelhoff, S
AU  - Edelhoff S
FAU - Disteche, C
AU  - Disteche C
FAU - Neubauer, M
AU  - Neubauer M
FAU - Marquardt, H
AU  - Marquardt H
AU  - et al.
LA  - eng
SI  - GENBANK/L19932
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - DNA Cell Biol
JT  - DNA and cell biology
JID - 9004522
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 148710-76-3 (betaIG-H3 protein)
RN  - 9007-49-2 (DNA)
SB  - IM
GS  - &bgr;ig-h3
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Cell Adhesion/physiology
MH  - Cell Division/physiology
MH  - Cell Transformation, Neoplastic
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 5
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA
MH  - *Extracellular Matrix Proteins
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - Molecular Sequence Data
MH  - Neoplasm Proteins/*genetics/physiology
MH  - Sequence Homology, Amino Acid
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1089/dna.1994.13.571 [doi]
PST - ppublish
SO  - DNA Cell Biol. 1994 Jun;13(6):571-84. doi: 10.1089/dna.1994.13.571.