PMID- 8025949
OWN - NLM
STAT- MEDLINE
DCOM- 19940811
LR  - 20141120
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 156
IP  - 2
DP  - 1994 Jul
TI  - Human polymorphonuclear leukocytes derived from chronically inflamed tissue 
      express inflammatory cytokines in vivo.
PG  - 296-309
AB  - In this study, we sought to determine if human polymorphonuclear leukocytes 
      (PMNs) derived from chronically inflamed tissues can produce inflammatory 
      cytokines in vivo. Human gingival crevicular fluid (GCF) with adult periodontitis 
      was collected, and PMNs in GCF were examined after purified by Ficoll-Hypaque 
      gradient method. Cytokines from peripheral blood (PB) cells stimulated with 
      concanavalin A, LPS, or zymosan were also characterized, since GCF contains 
      predominantly PMNs (> 95%) with a small number of lymphocytes or macrophages. 
      Production of interleukin-1 alpha (IL-1 alpha), IL-1 beta, tumor necrosis 
      factor-alpha (TNF alpha), and IL-6 in GCF or culture supernatants of peripheral 
      blood cells was determined by ELISA. Significant levels of IL-1 alpha and IL-1 
      beta secretion were found in GCF. PB cells in culture showed prominent cytokine 
      production from monocytes/macrophages, followed by lymphocytes. Human peripheral 
      blood PMNs (PB-PMNs) also produced low levels of IL-1 alpha and IL-1 beta, but 
      not TNF alpha and IL-6. These cells were also examined for cytokine mRNA 
      expression using the reverse transcription-polymerase chain reaction analysis. 
      Highly purified PMNs (> 99.5%) from GCF expressed mRNA for IL-1 alpha, IL-1 beta 
      and TNF alpha, but not for IL-6. PB-PMNs in culture also showed mRNA expression 
      for IL-1 alpha, IL-1 beta, and TNF alpha in a time- and dose-dependent manner, 
      especially after stimulation with zymosan. Therefore, we concluded that human 
      PMNs from inflamed tissues can produce IL-1 alpha, IL-1 beta, and TNF alpha in 
      vivo, but not IL-6.
FAU - Takeichi, O
AU  - Takeichi O
AD  - Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan.
FAU - Saito, I
AU  - Saito I
FAU - Tsurumachi, T
AU  - Tsurumachi T
FAU - Saito, T
AU  - Saito T
FAU - Moro, I
AU  - Moro I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Cytokines)
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Blood Cells/immunology
MH  - Cytokines/*biosynthesis/genetics
MH  - DNA Primers/genetics
MH  - DNA Probes/genetics
MH  - Gene Expression
MH  - Gingiva/immunology/pathology
MH  - Humans
MH  - In Vitro Techniques
MH  - Inflammation/*immunology
MH  - Interleukin-1/biosynthesis/genetics
MH  - Interleukin-6/biosynthesis/genetics
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Neutrophils/*immunology
MH  - Periodontitis/immunology/pathology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - S0008-8749(84)71176-2 [pii]
AID - 10.1006/cimm.1994.1176 [doi]
PST - ppublish
SO  - Cell Immunol. 1994 Jul;156(2):296-309. doi: 10.1006/cimm.1994.1176.