PMID- 8026827
OWN - NLM
STAT- MEDLINE
DCOM- 19940808
LR  - 20190722
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 25
IP  - 7
DP  - 1994 Jul
TI  - Comparison of fluorescence in situ hybridization with flow cytometry and static 
      image analysis in ploidy analysis of paraffin-embedded prostate adenocarcinoma.
PG  - 678-83
AB  - Nuclear DNA ploidy has been shown to have an important prognostic association for 
      patients with adenocarcinoma of the prostate. Flow cytometry and static image 
      analysis are ploidy methods that have been used in prostate carcinoma. 
      Fluorescence in situ hybridization (FISH) using chromosome-specific probes can be 
      used to evaluate the ploidy of interphase nuclei. In this study FISH was compared 
      with flow cytometry and static image analysis in determining ploidy in 
      paraffin-embedded tissue from 34 prostatic adenocarcinomas. Ploidy status using 
      FISH was determined by enumerating centromeres of two chromosomes (8 and 12) by 
      use of directly-labeled alpha-satellite DNA probes in isolated whole nuclei 
      obtained by the Hedley technique. All three methods identified 11 of 34 cases as 
      diploid and 17 of 34 cases as nondiploid (82% concordance). Six cases were 
      discordant; two cases had discrepant results by each method. Ploidy 
      classification as determined by FISH had an 88% concordance with ploidy 
      classification by either flow cytometry or static image analysis. In conclusion, 
      FISH was found to be a sensitive method of ploidy analysis in isolated 
      paraffin-embedded nuclei from prostate adenocarcinomas. When the chromosomes 
      commonly involved in aneuploidy have been identified in prostate adenocarcinoma, 
      FISH has the potential to provide greater sensitivity for aneuploidy detection 
      compared with currently available methods.
FAU - Persons, D L
AU  - Persons DL
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 
      55905.
FAU - Takai, K
AU  - Takai K
FAU - Gibney, D J
AU  - Gibney DJ
FAU - Katzmann, J A
AU  - Katzmann JA
FAU - Lieber, M M
AU  - Lieber MM
FAU - Jenkins, R B
AU  - Jenkins RB
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - DNA, Neoplasm/genetics
MH  - *Flow Cytometry
MH  - Humans
MH  - *Image Processing, Computer-Assisted
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Paraffin Embedding
MH  - Ploidies
MH  - Prostatic Neoplasms/*genetics
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 0046-8177(94)90301-8 [pii]
AID - 10.1016/0046-8177(94)90301-8 [doi]
PST - ppublish
SO  - Hum Pathol. 1994 Jul;25(7):678-83. doi: 10.1016/0046-8177(94)90301-8.