PMID- 8027772
OWN - NLM
STAT- MEDLINE
DCOM- 19940811
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 14
IP  - 7
DP  - 1994 Jul
TI  - Transforming growth factor alpha (TGF alpha) expression in degenerating 
      motoneurons of the murine mutant wobbler: a neuronal signal for astrogliosis?
PG  - 4206-16
AB  - The enhanced expression of the trophic factor transforming growth factor alpha 
      (TGF alpha) in reactive astrocytes following CNS injury suggests that TGF alpha 
      has a role in the development of astrogliosis. We explored this hypothesis in the 
      murine mutant wobbler, which presents a progressive motoneuronal degeneration 
      associated with an astrogliosis. Evolution of astrogliosis, and expression of TGF 
      alpha precursor (pro-TGF alpha) and of its receptor were examined over the course 
      of the disease, using genetically diagnosed animals and immunocytochemical 
      techniques. We report here that degenerating motoneurons of the cervical spinal 
      cord and a subset of astrocytes express pro-TGF alpha, prior to the onset of 
      astrogliosis, when the first clinical manifestations of the disease are observed 
      at 4 weeks of age. TGF alpha expression appeared strongly correlated with 
      motoneuronal degeneration. All pro-TGF alpha-immunoreactive neurons exhibited a 
      degenerative morphology, and the number of pro-TGF alpha-immunoreactive neurons 
      increased with the progression of the disease. At the glial level, we observed 
      that astrogliosis was a transitory phenomenon in the wobbler mice, developing in 
      coordination with the motoneuronal expression of pro-TGF alpha. Astrogliosis 
      became evident in 6-week-old wobbler mice, when the number of pro-TGF 
      alpha-immunoreactive motoneurons was maximal, and regressed in older mutant mice 
      in correlation with the disappearance of pro-TGF alpha-immunoreactive 
      motoneurons. Furthermore, TGF alpha/EGF receptor immunoreactivity was exclusively 
      localized in a subset of reactive astrocytes, its expression following closely 
      the course of the astrogliosis. These data show that TGF alpha synthesis by the 
      affected motoneurons is an early event in the course of the wobbler disease, and 
      suggest a role for TGF alpha as a neuronal inducer of astrocytic reactivity.
FAU - Junier, M P
AU  - Junier MP
AD  - CJF INSERM 91-02, Fac Medecine, Creteil, France.
FAU - Coulpier, M
AU  - Coulpier M
FAU - Le Forestier, N
AU  - Le Forestier N
FAU - Cadusseau, J
AU  - Cadusseau J
FAU - Suzuki, F
AU  - Suzuki F
FAU - Peschanski, M
AU  - Peschanski M
FAU - Dreyfus, P A
AU  - Dreyfus PA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Molecular Probes)
RN  - 0 (Transforming Growth Factor alpha)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Base Sequence
MH  - ErbB Receptors/metabolism
MH  - Gliosis/*metabolism
MH  - Mice
MH  - Mice, Neurologic Mutants
MH  - Molecular Probes/genetics
MH  - Molecular Sequence Data
MH  - Motor Neurons/*metabolism
MH  - Neck
MH  - *Nerve Degeneration
MH  - Polymerase Chain Reaction
MH  - Spinal Cord/metabolism
MH  - Time Factors
MH  - Transforming Growth Factor alpha/*metabolism
PMC - PMC6577032
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
PMCR- 1995/01/01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
PHST- 1995/01/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.14-07-04206.1994 [doi]
PST - ppublish
SO  - J Neurosci. 1994 Jul;14(7):4206-16. doi: 10.1523/JNEUROSCI.14-07-04206.1994.