PMID- 8029793
OWN - NLM
STAT- MEDLINE
DCOM- 19940808
LR  - 20141120
IS  - 0340-6245 (Print)
IS  - 0340-6245 (Linking)
VI  - 71
IP  - 3
DP  - 1994 Mar
TI  - Ex-vivo and in-vitro evidence that low molecular weight heparins exhibit less 
      binding to plasma proteins than unfractionated heparin.
PG  - 300-4
AB  - We have compared the non-specific binding of unfractionated heparin (UFH) with 
      that of low molecular weight heparin (LMWH) to plasma proteins both ex-vivo and 
      in-vitro. Non-specific binding to plasma proteins was assessed by comparing the 
      heparin levels measured as anti-factor Xa activity before and after the addition 
      of low affinity heparin, which is essentially devoid of anti-factor Xa activity, 
      in order to displace heparin bound to plasma proteins. For the ex-vivo studies, 
      we compared the recovery of UFH and a LMWH (ardeparin) from the plasma of 
      patients participating in a randomized trial of post-operative venous thrombosis 
      prophylaxis. For the in-vitro studies, we compared the recovery of UFH and 4 
      different LMWHs when added to the plasma from healthy volunteers and from 
      patients with suspected venous thromboembolic disease. The results indicate that 
      the recovery of LMWH is much less affected by nonspecific binding to plasma 
      proteins both ex-vivo and in-vitro. In addition, there are differences between 
      the LMWHs with respect to their plasma protein-binding.
FAU - Young, E
AU  - Young E
AD  - Hamilton Civic Hospitals Research Centre, Ontario, Canada.
FAU - Wells, P
AU  - Wells P
FAU - Holloway, S
AU  - Holloway S
FAU - Weitz, J
AU  - Weitz J
FAU - Hirsh, J
AU  - Hirsh J
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Thromb Haemost
JT  - Thrombosis and haemostasis
JID - 7608063
RN  - 0 (Blood Proteins)
RN  - 0 (Factor Xa Inhibitors)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Blood Proteins/*metabolism
MH  - Factor Xa Inhibitors
MH  - Heparin/*metabolism/pharmacology/therapeutic use
MH  - Heparin, Low-Molecular-Weight/*metabolism/pharmacology/therapeutic use
MH  - Humans
MH  - Molecular Weight
MH  - Neoplasms/blood/surgery
MH  - Postoperative Complications/prevention & control
MH  - Protein Binding
MH  - Thrombophlebitis/prevention & control
EDAT- 1994/03/01 00:00
MHDA- 1994/03/01 00:01
CRDT- 1994/03/01 00:00
PHST- 1994/03/01 00:00 [pubmed]
PHST- 1994/03/01 00:01 [medline]
PHST- 1994/03/01 00:00 [entrez]
PST - ppublish
SO  - Thromb Haemost. 1994 Mar;71(3):300-4.