PMID- 8030209
OWN - NLM
STAT- MEDLINE
DCOM- 19940811
LR  - 20071114
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 202
IP  - 2
DP  - 1994 Aug 1
TI  - N-linked oligosaccharides on herpes simplex virus glycoprotein gD are not 
      essential for establishment of viral latency or reactivation in the mouse eye 
      model.
PG  - 1050-3
AB  - Glycoprotein D (gD) is an essential component of the herpes simplex virus (HSV) 
      envelope. It is essential for viral penetration and for cell to cell spread of 
      virus in vitro, and is also important for neuroinvasiveness. We investigated the 
      contribution of N-linked oligosaccharides (N-CHO) on gD to viral pathogenesis. We 
      used F-gD(QAA), a mutant virus derived from strain F of HSV-1. This virus 
      contains three mutations in the gD gene which eliminate all signals for addition 
      of N-CHO. These mutations affect the antigenic structure of gD and also lead to a 
      small plaque phenotype. Otherwise the virus appears normal in in vitro assays. We 
      used the mouse eye model of HSV latency to examine whether the mutations alter 
      the phenotype of the virus in vivo. At 4 days postinfection similar amounts of 
      F-gD(QAA) and F-gD(WT), its wild-type parent, were found in either eyes or 
      trigeminal ganglia (TG) of infected mice. Moreover, both mutant and wild-type 
      viruses exhibited the same ability to establish, maintain, and be reactivated 
      from latency. We conclude that N-CHO on gD are not essential for HSV-1 
      pathogenesis in this model.
FAU - Tal-Singer, R
AU  - Tal-Singer R
AD  - Department of Microbiology, School of Dental Medicine, University of 
      Pennsylvania, Philadelphia 19104-6003.
FAU - Eisenberg, R J
AU  - Eisenberg RJ
FAU - Valyi-Nagy, T
AU  - Valyi-Nagy T
FAU - Fraser, N W
AU  - Fraser NW
FAU - Cohen, G H
AU  - Cohen GH
LA  - eng
GR  - AI-18289/AI/NIAID NIH HHS/United States
GR  - AI-23968/AI/NIAID NIH HHS/United States
GR  - DE-08239/DE/NIDCR NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Glycoconjugates)
RN  - 0 (RNA, Viral)
RN  - 0 (Receptors, Virus)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein D, Human herpesvirus 1)
SB  - IM
MH  - Animals
MH  - Eye/microbiology
MH  - Glycoconjugates/chemistry
MH  - In Situ Hybridization
MH  - Mice
MH  - RNA, Viral/genetics
MH  - Receptors, Virus/metabolism
MH  - Simplexvirus/*pathogenicity
MH  - Structure-Activity Relationship
MH  - Trigeminal Ganglion/microbiology
MH  - Viral Envelope Proteins/*chemistry
MH  - *Virus Latency
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - S0042-6822(84)71437-1 [pii]
AID - 10.1006/viro.1994.1437 [doi]
PST - ppublish
SO  - Virology. 1994 Aug 1;202(2):1050-3. doi: 10.1006/viro.1994.1437.