PMID- 8030228
OWN - NLM
STAT- MEDLINE
DCOM- 19940811
LR  - 20071114
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 202
IP  - 2
DP  - 1994 Aug 1
TI  - Defective measles virus in human subacute sclerosing panencephalitis brain.
PG  - 631-41
AB  - Evidence is presented showing that the brain cells of patients with subacute 
      sclerosing panencephalitis (SSPE) contain mutant measles (MV) genomes having the 
      characteristics of 5' copy-back defective interfering (DI) RNAs. Using a 
      polymerase chain reaction-based amplification specific for copy-back DIs, 
      abundant, discrete cDNAs representing different-sized MV defective RNA species 
      were generated from each SSPE brain. The defective genomes were cloned in two 
      portions. The most common of these defective species were sequenced, confirming 
      their MV genome origin and 5' copy-back nature. We deduced that the minimum DI 
      stem length of these species was 95 nucleotides, further delimiting the 
      prerequisite 5' regulatory region sequences specifying MV genomic 
      replication/encapsidation functions. This calculation assumes a precise copy-back 
      mechanism and complete complementarity of the panhandle structure. Since the 
      SSPE-derived viral genome encodes dysfunctional viral envelope proteins, we 
      hypothesize that SSPE brains may lack the high degree of selective pressure 
      encountered in tissue culture MV infections. This allows for the coexistence of 
      numerous replication-competent defective particles in each SSPE brain. A role for 
      viral defective particles as modulators of this persistent measles virus 
      infection of humans is proposed.
FAU - Sidhu, M S
AU  - Sidhu MS
AD  - Neurology Service, Department of Veterans Affairs Medical Center, East Orange, 
      New Jersey 07018.
FAU - Crowley, J
AU  - Crowley J
FAU - Lowenthal, A
AU  - Lowenthal A
FAU - Karcher, D
AU  - Karcher D
FAU - Menonna, J
AU  - Menonna J
FAU - Cook, S
AU  - Cook S
FAU - Udem, S
AU  - Udem S
FAU - Dowling, P
AU  - Dowling P
LA  - eng
GR  - AI35286/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA Primers)
RN  - 0 (Measles Vaccine)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Base Sequence
MH  - Brain/*microbiology
MH  - Cloning, Molecular
MH  - DNA Primers/chemistry
MH  - Defective Viruses/*genetics
MH  - Humans
MH  - In Situ Hybridization
MH  - Measles Vaccine/genetics
MH  - Measles virus/genetics/*pathogenicity
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/*genetics
MH  - Subacute Sclerosing Panencephalitis/*microbiology
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - S0042-6822(84)71384-5 [pii]
AID - 10.1006/viro.1994.1384 [doi]
PST - ppublish
SO  - Virology. 1994 Aug 1;202(2):631-41. doi: 10.1006/viro.1994.1384.