PMID- 8033102
OWN - NLM
STAT- MEDLINE
DCOM- 19940816
LR  - 20061115
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 54
IP  - 14
DP  - 1994 Jul 15
TI  - Fluorescence in situ hybridization analysis of 8p allelic loss and chromosome 8 
      instability in human prostate cancer.
PG  - 3824-30
AB  - Cytogenetic and molecular biological studies have demonstrated deletion of 
      sequences that map to the short arm of chromosome 8 (8p) in tumors from several 
      organ systems, including sequences that map within or near 8p22 in human prostate 
      tumors. Recent studies in our laboratory have suggested that deletion of 
      sequences on 8p may be concurrent with alterations in dosage for chromosome 8. In 
      order to further investigate this finding, the present study has applied 
      fluorescence in situ hybridization (FISH) techniques to determine the status of 
      chromosome 8 in prostate tumors that have undergone deletion of sequences at 
      8p22. Dosage of 8p22 sequences was assayed utilizing unique sequence cosmid DNA 
      probes by FISH and confirmed by amplification of microsatellite sequences by 
      polymerase chain reaction (PCR). Chromosome 8 dosage was assayed by FISH 
      utilizing both unique sequence cosmid probe DNA (specific to the 8q13.1-q13.3 
      chromosomal region) and pericentromeric probe DNA. FISH analysis of 10 specimens 
      of normal or benign prostatic hyperplasia tissues paired with 9 tumor and one 
      prostatic intraepithelial neoplasia tissues from the same patients for dosage at 
      8p, 8cen, and 8q, and PCR analysis for dosage at 8p, demonstrated that (a) FISH 
      provided a more precise means of evaluating allelic loss than PCR in prostate 
      tissue; (b) 8p22 sequence losses occurred frequently in prostate tumors; (c) 8p22 
      sequence losses were most often detected in the absence of 8cen or 8q sequence 
      dosage alterations, although they were sometimes accompanied by gain or loss of 
      8cen or 8q sequences; and (d) the pattern of 8p22 sequence losses was most often 
      widespread rather than focal. This study is the first to describe FISH analysis 
      of interphase nuclei within tissue sections using cosmid probe DNAs.
FAU - Macoska, J A
AU  - Macoska JA
AD  - Department of Urology, Wayne State University School of Medicine, Detroit, 
      Michigan 48201.
FAU - Trybus, T M
AU  - Trybus TM
FAU - Sakr, W A
AU  - Sakr WA
FAU - Wolf, M C
AU  - Wolf MC
FAU - Benson, P D
AU  - Benson PD
FAU - Powell, I J
AU  - Powell IJ
FAU - Pontes, J E
AU  - Pontes JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
SB  - IM
MH  - *Alleles
MH  - Base Sequence
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 8
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - Neoplasm Staging
MH  - Polymerase Chain Reaction
MH  - Prostatic Neoplasms/*genetics/pathology
EDAT- 1994/07/15 00:00
MHDA- 1994/07/15 00:01
CRDT- 1994/07/15 00:00
PHST- 1994/07/15 00:00 [pubmed]
PHST- 1994/07/15 00:01 [medline]
PHST- 1994/07/15 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1994 Jul 15;54(14):3824-30.