PMID- 8036172
OWN - NLM
STAT- MEDLINE
DCOM- 19940815
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 22
IP  - 12
DP  - 1994 Jun 25
TI  - Identification of a vitamin D responsive element in the promoter of the rat 
      cytochrome P450(24) gene.
PG  - 2410-6
AB  - Mitochondrial cytochrome P450(24) expression in the vitamin D-degradation pathway 
      is induced by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. The molecular basis of 
      this enzyme regulation was investigated by isolating the rat P450(24) gene and 
      examining the 5'-flanking region for possible cis-acting regulatory elements 
      involved in the induction process. Constructs containing different lengths of 
      5'-flanking region of the gene were linked to a luciferase reporter gene and 
      transiently co-transfected with a human vitamin D receptor (hVDR) expression 
      vector (pRSV-hVDR) into COS-1 cells. These experiments showed that the flanking 
      region from -298 to -122 directed a 24-fold increase in luciferase activity in 
      response to 1,25-(OH)2D3 provided that the cells were co-transfected with 
      pRSV-hVDR. Within this region, the sequence from position -171 to -123 conferred 
      1,25-(OH)2D3 responsiveness to both the native P450(24) promoter and the 
      heterologous thymidine kinase promoter. Mutagenesis revealed that the sequence 
      from position -150 to -136 is required for induction by 1,25-(OH)2D3 and that 
      this sequence shares similarity to other vitamin D responsive elements (VDREs) 
      reported for other genes. Gel shift mobility assays showed this region 
      specifically bound a nuclear protein complex from 1,25-(OH)2D3 treated COS-1 
      cells that had been co-transfected with pRSV-hVDR. The retarded band was 
      specifically competed with the well characterized VDRE from the mouse osteopontin 
      gene. A VDRE at position -150 to -136 in the promoter of the rat P450(24) gene is 
      identified in this study and found to be important in mediating the enhanced 
      expression of the gene by 1,25-(OH)2D3.
FAU - Hahn, C N
AU  - Hahn CN
AD  - Department of Biochemistry, University Adelaide, Australia.
FAU - Kerry, D M
AU  - Kerry DM
FAU - Omdahl, J L
AU  - Omdahl JL
FAU - May, B K
AU  - May BK
LA  - eng
SI  - GENBANK/Z28351
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 9007-49-2 (DNA)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.14.- (Steroid Hydroxylases)
RN  - EC 1.14.15.16 (Vitamin D3 24-Hydroxylase)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Calcitriol/*metabolism
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cytochrome P-450 Enzyme System/*genetics
MH  - DNA
MH  - *Gene Expression Regulation, Enzymologic
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Promoter Regions, Genetic
MH  - Rats
MH  - Repetitive Sequences, Nucleic Acid
MH  - Steroid Hydroxylases/*genetics
MH  - Transfection
MH  - Up-Regulation
MH  - Vitamin D3 24-Hydroxylase
PMC - PMC523703
EDAT- 1994/06/25 00:00
MHDA- 1994/06/25 00:01
CRDT- 1994/06/25 00:00
PHST- 1994/06/25 00:00 [pubmed]
PHST- 1994/06/25 00:01 [medline]
PHST- 1994/06/25 00:00 [entrez]
AID - 10.1093/nar/22.12.2410 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1994 Jun 25;22(12):2410-6. doi: 10.1093/nar/22.12.2410.