PMID- 8039157
OWN - NLM
STAT- MEDLINE
DCOM- 19940825
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 75
IP  - 1
DP  - 1994 Jul 1
TI  - Genetic alterations of chromosome 17 in human breast carcinoma studied by 
      fluorescence in situ hybridization and molecular DNA techniques.
PG  - 1-5
AB  - Fluorescence in situ hybridization (FISH) with a chromosome 17 
      centromere-specific probe was used together with Southern blot analyses and PCR 
      amplification of a polymorphic segment to characterize 13 breast carcinomas. 
      Cells with an abnormal number of chromosome 17 centromeres were found mixed with 
      disomic cells in nine of the 13 tumors studied. Three of the four cases found to 
      have a normal number of chromosome 17 centromeres had DNA alterations restricted 
      to allelic imbalance of the two most distal markers on 17p; the fourth had no 
      detectable alterations. In contrast, of the seven tumors found to have genetic 
      alterations elsewhere on chromosome 17, all had more than one cell population 
      when examined for numerical chromosome 17 alterations. The present data support 
      the hypothesis that alterations at the distal short arm of chromosome 17 
      represent earlier events in the tumorigenic process than do the other chromosome 
      17 abnormalities observed. The results of molecular DNA studies interpreted as 
      allelic imbalance of distal 17p markers in most cases probably reflect 
      chromosomal rearrangements like loss of specific loci, rather than monosomies and 
      large deletions. The report illustrates the usefulness of FISH when added to 
      current molecular DNA techniques.
FAU - Rosenberg, C
AU  - Rosenberg C
AD  - Department of Genetics, Norwegian Radium Hospital, Oslo.
FAU - Andersen, T I
AU  - Andersen TI
FAU - Nesland, J M
AU  - Nesland JM
FAU - Lier, M E
AU  - Lier ME
FAU - Brogger, A
AU  - Brogger A
FAU - Borresen, A L
AU  - Borresen AL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 17
MH  - DNA, Neoplasm/*analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 0165-4608(94)90207-0 [pii]
AID - 10.1016/0165-4608(94)90207-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1994 Jul 1;75(1):1-5. doi: 10.1016/0165-4608(94)90207-0.