PMID- 8039162
OWN - NLM
STAT- MEDLINE
DCOM- 19940825
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 75
IP  - 1
DP  - 1994 Jul 1
TI  - Evaluation of 20 archival prostate tumor specimens by fluorescence in situ 
      hybridization (FISH).
PG  - 40-4
AB  - Analysis of 20 primary prostatic tumors (local Gleason grades ranging from 1 to 
      4) was performed on archival material obtained from formalin-fixed, 
      paraffin-embedded blocks. Alpha satellite probes specific for the pericentric 
      regions of chromosomes 12, 17, X, and Y were used in fluorescence in situ 
      hybridization (FISH) assays for the examination of aneusomies for these 
      chromosomes. Eighty percent of specimens (16/20) showed significant loss of 
      chromosome 17 and 55% (11/20 specimens) also showed significant loss of 
      chromosome 12; all specimens that lost chromosome 12 lost chromosome 17. Gain of 
      the X chromosome was observed in 40% (8/20) of specimens, all but one of which 
      also showed loss of chromosome 17. While the specimens showing gain of the X 
      chromosome may represent polyploid cells, only one specimen also showed 
      significant gain of chromosomes 12 and 17, suggesting that both of these 
      chromosomes may be lost in hyperdiploid prostate cancers. Loss of the Y 
      chromosome was not statistically significant. This study thus indicates that loss 
      of chromosome 17 is a frequent and likely early event in prostatic cancer.
FAU - Brothman, A R
AU  - Brothman AR
AD  - Department of Human Genetics, University of Utah School of Medicine, Salt Lake 
      City 84112.
FAU - Watson, M J
AU  - Watson MJ
FAU - Zhu, X L
AU  - Zhu XL
FAU - Williams, B J
AU  - Williams BJ
FAU - Rohr, L R
AU  - Rohr LR
LA  - eng
GR  - R01-CA46269/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 12
MH  - Chromosomes, Human, Pair 17
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Prostatic Neoplasms/*genetics
MH  - Sex Chromosomes
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 0165-4608(94)90213-5 [pii]
AID - 10.1016/0165-4608(94)90213-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1994 Jul 1;75(1):40-4. doi: 10.1016/0165-4608(94)90213-5.