PMID- 8041631
OWN - NLM
STAT- MEDLINE
DCOM- 19940824
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 22
IP  - 13
DP  - 1994 Jul 11
TI  - Translational enhancement of H-ferritin mRNA by interleukin-1 beta acts through 
      5' leader sequences distinct from the iron responsive element.
PG  - 2678-86
AB  - Interleukin-1 beta (Il-1 beta), a key cytokine in the acute phase response, 
      elevates hepatic expression of both the heavy (H) and light (L) ferritin subunits 
      without influencing the steady-state levels of either ferritin transcript. 
      Transfection experiments with human hepatoma cells reveal that sequences within 
      the 5' untranslated region (5'UTR) of H-ferritin mRNA confer translational 
      regulation to chimaeric chloramphenicol acetyl transferase (CAT) mRNAs in 
      response to Il-1 beta in the absence of marked changes in CAT mRNA levels. Il-1 
      beta dependent translational enhancement is mediated by a distinct G + C rich RNA 
      sequence within 70 nucleotides (nt) of the start codon. The upstream Iron 
      Responsive Element RNA stemloop does not confer increased expression to CAT mRNA 
      in Il-1 beta stimulated hepatoma transfectants. A 38 nucleotide consensus 
      sequence within the 5'UTRs of the mRNAs encoding the hepatic acute phase proteins 
      alpha 1-antitrypsin (alpha 1AT), alpha 1-acid glycoprotein (AGP) and haptoglobin 
      (Dente et al., 1985) is similar to sequences in the G + C rich H-ferritin mRNA 
      translational regulatory element. Deletion of three nucleotides from this region 
      of the 61 nt G + C rich element in the H-ferritin mRNA 5' leader eliminates Il-1 
      beta translational enhancement of the CAT reporter transcripts.
FAU - Rogers, J T
AU  - Rogers JT
AD  - Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 
      Boston, MA 02115.
FAU - Andriotakis, J L
AU  - Andriotakis JL
FAU - Lacroix, L
AU  - Lacroix L
FAU - Durmowicz, G P
AU  - Durmowicz GP
FAU - Kasschau, K D
AU  - Kasschau KD
FAU - Bridges, K R
AU  - Bridges KR
LA  - eng
GR  - R01 HL45794/HL/NHLBI NIH HHS/United States
GR  - R29 AI32717-01/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
RN  - 9007-73-2 (Ferritins)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Base Sequence
MH  - Consensus Sequence
MH  - DNA
MH  - Ferritins/chemistry/*genetics/metabolism
MH  - Humans
MH  - Interleukin-1/*physiology
MH  - Iron/metabolism
MH  - Molecular Sequence Data
MH  - *Protein Biosynthesis
MH  - RNA, Messenger/metabolism
MH  - *Regulatory Sequences, Nucleic Acid
MH  - Transfection
MH  - Tumor Cells, Cultured
PMC - PMC308227
EDAT- 1994/07/11 00:00
MHDA- 1994/07/11 00:01
CRDT- 1994/07/11 00:00
PHST- 1994/07/11 00:00 [pubmed]
PHST- 1994/07/11 00:01 [medline]
PHST- 1994/07/11 00:00 [entrez]
AID - 10.1093/nar/22.13.2678 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1994 Jul 11;22(13):2678-86. doi: 10.1093/nar/22.13.2678.