PMID- 8042271
OWN - NLM
STAT- MEDLINE
DCOM- 19940823
LR  - 20190830
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 39
IP  - 3-4
DP  - 1994 Apr
TI  - Detection of Actinobacillus pleuropneumoniae serotype 2 antibodies in pig sera by 
      an inhibition enzyme immuno assay (EIA).
PG  - 231-44
AB  - An inhibition Enzyme Immuno Assay (EIA) for detection of antibodies against A. 
      pleuropneumoniae serotype 2 (App-2) in pig sera, based on the inhibition of the 
      binding of an App-2 specific monoclonal antibody was established. The monoclonal 
      antibody (mAb 102-G02) was found to be directed against an epitope on the O-chain 
      of App-2 LPS. Some App-2 isolates did not react with the mAb 102-G02. These 
      isolates are referred to as App-2X. In the inhibition EIA highly purified App-2 
      LPS ws used to coat microtiter plates. Serial dilutions of pig sera were added to 
      the plates prior to the mAb 102-G02. The degree of binding of App-2 antibodies 
      from pig sera was determined as the percentage inhibition of the mAb 102-G02. Pig 
      sera from specific pathogen free (SPF) herds, from experimentally infected 
      animals, and from conventionel herds were tested. A serum dilution of 1/200 was 
      found to be optimal, when using 50% inhibition as the discriminating inhibition 
      percentage. Serum from App-2X infected herds showed a lower reactivity as 
      compared to serum from App-2 infected herds. No crossreactivity was observed with 
      serum from pigs infected with other App serotypes. The sensitivity and 
      specificity were 100% and 98.9%, respectively.
FAU - Stenbaek, E I
AU  - Stenbaek EI
AD  - National Veterinary Laboratory, Copenhagen, Denmark.
FAU - Schirmer, A L
AU  - Schirmer AL
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Immune Sera)
SB  - IM
MH  - Actinobacillus Infections/diagnosis/*veterinary
MH  - Actinobacillus pleuropneumoniae/*immunology
MH  - Animals
MH  - Antibodies, Bacterial/*blood
MH  - Antibodies, Monoclonal/biosynthesis/immunology
MH  - Complement Fixation Tests
MH  - Cross Reactions
MH  - Immune Sera/immunology
MH  - Immunoenzyme Techniques/veterinary
MH  - Mice
MH  - Pleuropneumonia/diagnosis/*veterinary
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Specific Pathogen-Free Organisms
MH  - Swine
MH  - Swine Diseases/*diagnosis
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - 0378-1135(94)90160-0 [pii]
AID - 10.1016/0378-1135(94)90160-0 [doi]
PST - ppublish
SO  - Vet Microbiol. 1994 Apr;39(3-4):231-44. doi: 10.1016/0378-1135(94)90160-0.