PMID- 8043947
OWN - NLM
STAT- MEDLINE
DCOM- 19940901
LR  - 20190904
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 5
IP  - 6
DP  - 1994 Jun
TI  - The mapping of transgenes by fluorescence in situ hybridization on G-banded mouse 
      chromosomes.
PG  - 337-41
AB  - A highly sensitive method for the mapping of transgenes and other genes in the 
      mouse genome is described. This technique combines high-resolution G-banding and 
      fluorescence in situ hybridization (FISH) with either biotin/avidin-FITC or 
      digoxigenin-anti-digoxigenin-FITC, the latter being the more sensitive. Banding 
      patterns are obtained with trypsin/Giemsa-treated slides, and sensitivity is 
      greatly increased by the use of mouse Cot-1 DNA. With this protocol, four 
      different 14.5-kb human Cu/Zn-superoxide dismutase transgene insertions ranging 
      in copy number from 2 to 8 have been localized to four different mouse 
      chromosomes. The utility and sensitivity of this procedure were verified with a 
      Chromosome (Chr) 16-specific cosmid probe, H22, as well as with the mapping of a 
      high-copy-number human beta-amyloid/A4 transgene.
FAU - Shi, Y P
AU  - Shi YP
AD  - Department of Pediatrics, University of California, San Francisco 94143.
FAU - Huang, T T
AU  - Huang TT
FAU - Carlson, E J
AU  - Carlson EJ
FAU - Epstein, C J
AU  - Epstein CJ
LA  - eng
GR  - AG-08938/AG/NIA NIH HHS/United States
GR  - HD-03132/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA Probes)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Chromosome Banding/methods
MH  - Chromosome Mapping/*methods
MH  - Cosmids
MH  - DNA Probes
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, Transgenic/*genetics
MH  - Superoxide Dismutase/genetics
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1007/BF00356551 [doi]
PST - ppublish
SO  - Mamm Genome. 1994 Jun;5(6):337-41. doi: 10.1007/BF00356551.