PMID- 8044650
OWN - NLM
STAT- MEDLINE
DCOM- 19940831
LR  - 20190515
IS  - 1018-4813 (Print)
IS  - 1018-4813 (Linking)
VI  - 1
IP  - 3
DP  - 1993
TI  - A simple method for prenatal diagnosis of trisomy 21 on uncultured amniocytes.
PG  - 245-51
AB  - Prenatal diagnosis of trisomy 21 would be easier if fluorescence in situ 
      hybridization (FISH) could be applied to interphase nuclei. Therefore, we 
      prepared a chromosome-21-specific probe by in vitro enzymatic amplification of 
      inter-Alu sequences from YAC clones previously localized to this chromosome. This 
      probe was used for FISH on 22 uncultured amniocyte samples. An easy, rapid, and 
      safe technique is proposed for the prenatal diagnosis of trisomy 21.
FAU - Romana, S P
AU  - Romana SP
AD  - INSERM U301, Institut de Genetique Moleculaire, Paris, France.
FAU - Tachdjian, G
AU  - Tachdjian G
FAU - Druart, L
AU  - Druart L
FAU - Cohen, D
AU  - Cohen D
FAU - Berger, R
AU  - Berger R
FAU - Cherif, D
AU  - Cherif D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Hum Genet
JT  - European journal of human genetics : EJHG
JID - 9302235
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Amniocentesis/*methods
MH  - Base Sequence
MH  - *Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 21
MH  - DNA Primers
MH  - *DNA Probes
MH  - Down Syndrome/*diagnosis/genetics
MH  - Female
MH  - Fetal Diseases/diagnosis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interphase
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/methods
MH  - Pregnancy
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1159/000472418 [doi]
PST - ppublish
SO  - Eur J Hum Genet. 1993;1(3):245-51. doi: 10.1159/000472418.