PMID- 8045422
OWN - NLM
STAT- MEDLINE
DCOM- 19940831
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 145
IP  - 1
DP  - 1994 Jul 22
TI  - Primary structure and biological features of a thermostable nuclease isolated 
      from Staphylococcus hyicus.
PG  - 41-7
AB  - The nucH gene, encoding a thermostable nuclease (TNase), was isolated from the 
      cellular DNA of Staphylococcus hyicus strain E80 and sequenced. NucH, the 
      169-amino-acid (aa) protein encoded by this gene, contains, at its N-terminus, a 
      signal peptide which appears to be cleaved at the same site in S. hyicus and 
      Escherichia coli, yielding a mature protein which is exported extracellularly 
      from S. hyicus, but not from E. coli. The aa sequence of NucH is highly 
      homologous with that of the TNase from S. intermedius strain LRA076, whereas 
      significant similarities are observed with the TNase from S. aureus, as well as 
      with three other bacterial proteins of which only one has been shown to exhibit 
      DNase activity. As seen in a multiple sequence alignment, the invariant residues 
      are mostly located in the regions involved in the biological activity of the S. 
      aureus TNase. The ability of crude cell extracts of E. coli strains carrying nucH 
      to degrade various forms of nucleic acids with or without Ca2+ supplementation 
      was studied. Under our experimental conditions, the enzyme encoded by nucH was 
      active at 37 degrees C on both DNA and RNA, had the potential to act as an 
      endonuclease, and functioned in the presence of Ca2+. Moreover, activity was 
      retained after heating at 100 degrees C, suggesting that the enzyme could undergo 
      reversible unfolding.
FAU - Chesneau, O
AU  - Chesneau O
AD  - National Reference Center for Staphylococci, Laboratoire des Staphylocoques et 
      des Streptocoques, Institut Pasteur, Paris, France.
FAU - el Solh, N
AU  - el Solh N
LA  - eng
SI  - GENBANK/L23973
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Bacterial)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
SB  - IM
GS  - nucH
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - DNA, Bacterial
MH  - Gene Expression Regulation, Bacterial
MH  - Micrococcal Nuclease/chemistry/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Restriction Mapping
MH  - Sequence Homology, Amino Acid
MH  - Staphylococcus/*enzymology/genetics
EDAT- 1994/07/22 00:00
MHDA- 1994/07/22 00:01
CRDT- 1994/07/22 00:00
PHST- 1994/07/22 00:00 [pubmed]
PHST- 1994/07/22 00:01 [medline]
PHST- 1994/07/22 00:00 [entrez]
AID - 0378-1119(94)90320-4 [pii]
AID - 10.1016/0378-1119(94)90320-4 [doi]
PST - ppublish
SO  - Gene. 1994 Jul 22;145(1):41-7. doi: 10.1016/0378-1119(94)90320-4.