PMID- 8045461
OWN - NLM
STAT- MEDLINE
DCOM- 19940830
LR  - 20190830
IS  - 0278-6915 (Print)
IS  - 0278-6915 (Linking)
VI  - 32
IP  - 6
DP  - 1994 Jun
TI  - The performance of the local lymph node assay with chemicals identified as 
      contact allergens in the human maximization test.
PG  - 543-7
AB  - For many years, tests in the guinea pig have been the favoured option for the 
      identification of the skin sensitization potential of chemicals. However, the 
      mouse has been used widely in immunology research and can represent a viable 
      alternative. A variety of murine assays have been described, including several 
      methods based on ear swelling as an endpoint. Another option is to assess induced 
      lymph node cell proliferation and it is this which forms the basis of the murine 
      local lymph node assay (LLNA). The LLNA has undergone several successful 
      interlaboratory validations and compares well with standard guinea pig assays. In 
      the present study, the performance of the LLNA was examined with chemicals tested 
      previously in the human maximization test (HMT). 30 chemicals, 23 of which proved 
      positive in the HMT and seven of which were negative, have been tested. All but 
      four of the materials found positive in the HMT also tested positive in the LLNA. 
      Of these four, sulfanilamide and paraben esters would not classify as skin 
      sensitizers in the guinea pig maximization test and nickel has been found to 
      yield variable results in a number of predictive animal tests. Of the seven 
      substances which proved negative in the HMT, six were also negative in the LLNA, 
      the exception being sodium dodecyl sulfate. These data demonstrate that the LLNA 
      is able to identify accurately chemicals which have the potential to cause 
      significant allergic contact dermatitis in humans.
FAU - Basketter, D A
AU  - Basketter DA
AD  - Unilever Environmental Safety Laboratory, Sharnbrook, Bedford, UK.
FAU - Scholes, E W
AU  - Scholes EW
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/immunology/*toxicity
MH  - Animals
MH  - Cell Division/drug effects
MH  - Dermatitis, Allergic Contact/*etiology
MH  - Female
MH  - Humans
MH  - Lymph Nodes/cytology/*drug effects/immunology
MH  - Mice
MH  - Mice, Inbred CBA
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 0278-6915(94)90112-0 [pii]
AID - 10.1016/0278-6915(94)90112-0 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 1994 Jun;32(6):543-7. doi: 10.1016/0278-6915(94)90112-0.