PMID- 8045842
OWN - NLM
STAT- MEDLINE
DCOM- 19940830
LR  - 20171213
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 76
IP  - 4
DP  - 1994 Apr
TI  - Vitamin E effects on indexes of lipid peroxidation in muscle from DHEA-treated 
      and exercised rats.
PG  - 1630-5
AB  - Sixty-four male Sprague-Dawley rats were randomly assigned to one of eight 
      treatment groups to determine the effects of vitamin E (VitE), 
      dehydroepiandrosterone (DHEA), and exercise on antioxidant status in plasma and 
      skeletal muscle. Indexes of oxidative stress were determined by measuring two 
      markers of lipid peroxidation and the activity of two free radical scavenging 
      enzymes. One-half of the rats had their diets supplemented with 250 IU VitE/kg of 
      diet. One-half of the rats were injected with 0.35 mol/kg body wt ip of 
      DHEA-acetate, whereas the others were injected with vehicle. All treatments 
      lasted 5 wk. Before being killed, one-half of each treatments group of rats was 
      randomly assigned to run for 1 h on a motorized rodent treadmill at 21 m/min up a 
      12% grade. The other rats remained rested before being killed. Exercise increased 
      thiobarbituric acid-reactive substances (TBARS) and lipid hydroperoxides in 
      plasma and TBARS in red slow-twitch and white fast-twitch muscles. VitE reduced 
      the amount of lipid hydroperoxides and TBARS in plasma and TBARS in all three 
      muscle fiber types. VitE also reduced glutathione peroxidase (GPX) activity in 
      plasma and red fast-twitch muscle. DHEA increased indexes of oxidative stress in 
      plasma and white fast-twitch muscle. DHEA reduced GPX activity in plasma but 
      increased GPX activity in all three muscle fiber types. These results indicate 
      that aerobic exercise is a mild oxidative stressor with DHEA exacerbating this 
      response and that VitE helps diminish this effect in certain muscle fiber types.
FAU - Goldfarb, A H
AU  - Goldfarb AH
AD  - Department of Exercise and Sport Science, University of North Carolina at 
      Greensboro 27412.
FAU - McIntosh, M K
AU  - McIntosh MK
FAU - Boyer, B T
AU  - Boyer BT
FAU - Fatouros, J
AU  - Fatouros J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 1406-18-4 (Vitamin E)
RN  - 459AG36T1B (Dehydroepiandrosterone)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
SB  - IM
MH  - Adipose Tissue/drug effects/physiology
MH  - Animals
MH  - Body Weight/drug effects/physiology
MH  - Catalase/metabolism
MH  - Dehydroepiandrosterone/*pharmacology
MH  - Glutathione Peroxidase/blood
MH  - Lipid Peroxidation/*drug effects
MH  - Male
MH  - Muscles/drug effects/enzymology/*metabolism
MH  - Oxidation-Reduction
MH  - *Physical Conditioning, Animal
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Vitamin E/blood/*pharmacology
EDAT- 1994/04/01 00:00
MHDA- 1994/04/01 00:01
CRDT- 1994/04/01 00:00
PHST- 1994/04/01 00:00 [pubmed]
PHST- 1994/04/01 00:01 [medline]
PHST- 1994/04/01 00:00 [entrez]
AID - 10.1152/jappl.1994.76.4.1630 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 1994 Apr;76(4):1630-5. doi: 10.1152/jappl.1994.76.4.1630.