PMID- 8046327
OWN - NLM
STAT- MEDLINE
DCOM- 19940830
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 180
IP  - 2
DP  - 1994 Aug 1
TI  - Leukocyte common antigen (CD45) is required for immunoglobulin E-mediated 
      degranulation of mast cells.
PG  - 471-6
AB  - We demonstrate using primary mast cell cultures derived from wild-type and 
      CD45-deficient mice that mast cell triggering through the high-affinity 
      immunoglobulin E (IgE) receptor requires the cell surface tyrosine phosphatase 
      CD45. Unlike wild-type cells, cross-linking of surface-bound IgE in mast cells 
      deficient in CD45 does not induce degranulation. Degranulation in these mutant 
      cells does occur after treatment with the calcium ionophore A23187 indicating 
      that the degranulation machinery is intact in these cells. We also demonstrate 
      that the tyrosine phosphatase inhibitors orthoVanadate and perVanadate inhibit 
      degranulation in wild-type mast cells, as does cross-linking of CD45 by anti-CD45 
      antibodies. Finally, we show that CD45-deficient mice are resistant to 
      IgE-dependent systemic anaphylaxis. These results show that, like the T cell 
      receptor and the antigen receptor on B cells, there is an absolute requirement 
      for CD45 in signaling via the high affinity IgE receptor, expanding the number of 
      receptors for which CD45 is an essential component.
FAU - Berger, S A
AU  - Berger SA
AD  - Wellesley Hospital Research Institute, Toronto, Ontario, Canada.
FAU - Mak, T W
AU  - Mak TW
FAU - Paige, C J
AU  - Paige CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 3WHH0066W5 (Vanadates)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Anaphylaxis/immunology
MH  - Animals
MH  - B-Lymphocytes/immunology
MH  - Calcimycin/pharmacology
MH  - *Cell Degranulation
MH  - Cell Line
MH  - Flow Cytometry
MH  - Immunoglobulin E/*immunology
MH  - Leukocyte Common Antigens/*immunology
MH  - Mast Cells/*immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mutation
MH  - Receptors, Antigen, T-Cell/immunology/metabolism
MH  - Vanadates/pharmacology
PMC - PMC2191589
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
PMCR- 1995/02/01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
PHST- 1995/02/01 00:00 [pmc-release]
AID - 94321901 [pii]
AID - 10.1084/jem.180.2.471 [doi]
PST - ppublish
SO  - J Exp Med. 1994 Aug 1;180(2):471-6. doi: 10.1084/jem.180.2.471.