PMID- 8046393
OWN - NLM
STAT- MEDLINE
DCOM- 19940830
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 75 ( Pt 8)
DP  - 1994 Aug
TI  - Critical involvement of human T cell leukaemia virus type I virions in mediating 
      the viral mitogenic effect.
PG  - 1909-16
AB  - Human T cell leukaemia virus type I (HTLV-I) is a direct activator of human 
      resting T lymphocytes. The present study was undertaken to delineate further the 
      role of viral particles and to define the involvement of envelope glycoproteins 
      in the induction of T cell mitogenic stimulation. Virus-producing cells treated 
      with paraformaldehyde (PFA) were found to be unable to induce the formation of 
      syncytia, but still able to trigger the proliferation of resting T cells. 
      Likewise, PFA-treated virus particles were still mitogenic. These results suggest 
      that the mitogenic event is triggered before the fusion of the envelope with the 
      cell membrane. Furthermore, HTLV-I envelope-expressing cells obtained after 
      infection of C8166/45 cells (HTLV-I-transformed, but defective in virion 
      production) with an HTLV-I envelope recombinant vaccinia virus were unable to 
      activate normal T cells. Human immuno-deficiency virus type 1 particles produced 
      by C8166/45 cells were also devoid of mitogenic ability. However, when HTLV-I 
      viral preparations were purified by chromatography, only the virion-containing 
      fractions were found to be mitogenic for human resting T lymphocytes. This 
      mitogenic activity was partially abolished by preincubating the purified virus 
      with a monoclonal antibody directed to the surface envelope glycoprotein. 
      Finally, treatment of HTLV-I-transformed cells by tunicamycin, an inhibitor of 
      N-linked glycosylation, led to the production of virus particles with a decreased 
      mitogenic activity. Collectively, these observations suggest that the HTLV-I 
      mitogenic activity is triggered by the contact of HTLV-I virions with T cells.
FAU - Casse, H
AU  - Casse H
AD  - Centre National de la Recherche Scientifique, Universite Claude Bernard, Faculte 
      de Medecine A. Carrel, Lyon, France.
FAU - Girerd, Y
AU  - Girerd Y
FAU - Gazzolo, L
AU  - Gazzolo L
FAU - Duc Dodon, M
AU  - Duc Dodon M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Mitogens)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Cell Division
MH  - Human T-lymphotropic virus 1/*immunology
MH  - Humans
MH  - *Lymphocyte Activation
MH  - Mitogens
MH  - Recombinant Proteins/immunology
MH  - T-Lymphocytes/*immunology
MH  - Viral Envelope Proteins/genetics/immunology
MH  - Virion/*immunology
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
AID - 10.1099/0022-1317-75-8-1909 [doi]
PST - ppublish
SO  - J Gen Virol. 1994 Aug;75 ( Pt 8):1909-16. doi: 10.1099/0022-1317-75-8-1909.