PMID- 8046543
OWN - NLM
STAT- MEDLINE
DCOM- 19940901
LR  - 20190512
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 8
IP  - 3
DP  - 1994
TI  - Anti-HTLV-I IgG in urine detected by sensitive enzyme immunoassay (immune complex 
      transfer enzyme immunoassay) using a synthetic peptide, Cys-env gp46(188-224), as 
      antigen.
PG  - 149-56
AB  - Antibody IgG to human T-cell leukemia virus type I (HTLV-I) in urine was detected 
      by a sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) 
      using a synthetic peptide, Cys-env gp46(188-224), as antigen, the sensitivity and 
      specificity of which were 100 and 98.5%, respectively, using serum samples. 
      Anti-HTLV-I IgG in urine was reacted simultaneously with 2,4-dinitrophenyl-bovine 
      serum albumin-Cys-env gp46(188-224) conjugate and Cys-env 
      gp46(188-224)-beta-D-galactosidase (Escherichia coli) conjugate. The complex 
      formed, consisting of the three components, was trapped onto polystyrene balls 
      coated with affinity-purified (anti-2,4-dinitrophenyl group) IgG, eluted with 
      epsilon N-2,4-dinitrophenyl-L-lysine and transferred to polystyrene balls coated 
      with affinity-purified (anti-human IgG gamma-chain) IgG. Finally, bound 
      beta-D-galactosidase activity was assayed by fluorometry. Thirty-one urine 
      samples from seropositive subjects and 100 urine samples from seronegative 
      subjects were tested. The sensitivity and specificity were 87 and 100%, 
      respectively, with unconcentrated urine samples and 94 and 100%, respectively, 
      with approximately 10-fold concentrated urine samples. These results were 
      superior to those by the conventional ELISA and gelatin particle agglutination 
      test.
FAU - Hashida, S
AU  - Hashida S
AD  - Department of Biochemistry, Medical College of Miyazaki, Japan.
FAU - Hirota, K
AU  - Hirota K
FAU - Kohno, T
AU  - Kohno T
FAU - Ishikawa, E
AU  - Ishikawa E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (HTLV-I Antigens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen-Antibody Complex/metabolism
MH  - Female
MH  - HTLV-I Antibodies/*urine
MH  - HTLV-I Antigens/*immunology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunoglobulin G/*urine
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Peptide Fragments/*immunology
MH  - Rabbits
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1002/jcla.1860080307 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 1994;8(3):149-56. doi: 10.1002/jcla.1860080307.