PMID- 8047979
OWN - NLM
STAT- MEDLINE
DCOM- 19940830
LR  - 20181130
IS  - 0039-6060 (Print)
IS  - 0039-6060 (Linking)
VI  - 116
IP  - 2
DP  - 1994 Aug
TI  - Sepsis-induced release of interleukin-6 may activate the immediate-early gene 
      program through a hypothalamic-hypophyseal mechanism.
PG  - 141-8; discussion 148-9
AB  - BACKGROUND: The immediate-early gene c-fos has been implicated in transcriptional 
      regulation after sepsis. We test the hypothesis that sepsis-induced central 
      nervous system release of interleukin (IL)-6 regulates hepatic c-fos gene 
      expression. METHODS: Using a stereotaxically placed intracerebral-ventricular 
      (ICV) catheter in rats with and without hypophysectomy, we measured hepatic c-fos 
      protein accumulation after treatment with either IL-6 or vehicle control. Using a 
      rat cecal ligation and puncture (CLP) model, we studied the following groups: (1) 
      sham-CLP, (2) CLP, (3) hypophysectomized sham-CLP, and (4) hypophysectomized CLP 
      and measured hepatic c-fos mRNA. RESULTS: ICV IL-6 treatment increased hepatic 
      c-fos protein in the IL-6-treated group compared with the vehicle-treated group, 
      and hypophysectomy inhibited the ICV IL-6-mediated increase in c-fos protein. 
      After peritoneal sepsis, CLP increased hepatic c-fos messenger RNA compared to 
      either the sham-CLP or the hypophysectomized sham-CLP group, and hypophysectomy 
      before CLP inhibited hepatic c-fos mRNA compared with the CLP group. CONCLUSIONS: 
      ICV IL-6 results in an increase in hepatic fos protein that is mediated through a 
      hypothalamic-hypophyseal mechanism. Peritoneal sepsis results in an increase in 
      hepatic c-fos gene expression that may be, in part, mediated by central nervous 
      system release of IL-6 through a hypothalamic-hypophyseal mechanism.
FAU - Barke, R A
AU  - Barke RA
AD  - Department of Surgery, University of Minnesota, Minneapolis.
FAU - Roy, S
AU  - Roy S
FAU - Chapin, R B
AU  - Chapin RB
FAU - Charboneau, R
AU  - Charboneau R
FAU - Brady, P S
AU  - Brady PS
FAU - Brady, L J
AU  - Brady LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Surgery
JT  - Surgery
JID - 0417347
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Bacterial Infections/*metabolism
MH  - Base Sequence
MH  - *Gene Expression Regulation
MH  - *Genes, Immediate-Early
MH  - *Genes, fos
MH  - Hypothalamo-Hypophyseal System/*physiology
MH  - Interleukin-6/*metabolism
MH  - Male
MH  - Molecular Sequence Data
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1994/08/01 00:00
MHDA- 1994/08/01 00:01
CRDT- 1994/08/01 00:00
PHST- 1994/08/01 00:00 [pubmed]
PHST- 1994/08/01 00:01 [medline]
PHST- 1994/08/01 00:00 [entrez]
PST - ppublish
SO  - Surgery. 1994 Aug;116(2):141-8; discussion 148-9.