PMID- 8048538
OWN - NLM
STAT- MEDLINE
DCOM- 19940830
LR  - 20180109
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 267
IP  - 1 Pt 1
DP  - 1994 Jul
TI  - 3'-untranslated region of SP-B mRNA mediates inhibitory effects of TPA and 
      TNF-alpha on SP-B expression.
PG  - L16-24
AB  - Surfactant protein-B (SP-B) is a small hydrophobic polypeptide that enhances 
      spreading and stability of surfactant phospholipids in the alveolus of the lung. 
      Decreased expression of SP-B is associated with respiratory failure in premature 
      infants and in adult patients with acute respiratory distress syndrome (ARDS). 
      Tumor necrosis factor-alpha (TNF-alpha) and 12-O-tetradecanoylphorbol-13 acetate 
      (TPA) cause ARDS-like lung injury in vivo. Inhibitory effects of TPA and 
      TNF-alpha on SP-B mRNA expression in vitro were mediated by decreased SP-B mRNA 
      stability rather than by decreased rate of SP-B gene transcription. In the 
      present study, a human pulmonary adenocarcinoma cell line, NCI H441-4, was stably 
      transfected with expression vectors consisting of the thymidine kinase (TK) 
      promotor and human growth hormone (hGH) gene, in which the hGH 3'-untranslated 
      region (3'-UTR) was replaced by the 2.0-kb human SP-B cDNA [pTKGH(SP-B2.0)] or 
      the 837-bp human SP-B 3'-UTR [pTKGH(SP-B.837)]. The mRNAs and cellular growth 
      hormone protein generated from the chimeric TKGH(SP-B2.0) and TKGH(SP-B.837) 
      genes were each inhibited by approximately 50% by TPA and TNF-alpha. 
      Dexamethasone decreased the inhibitory effects of TPA and TNF-alpha. The 
      inhibition of steady-state hGH-SP-B mRNA by TPA and TNF-alpha was mediated by a 
      cis-active element located in the 3-UTR region of SP-B mRNA.
FAU - Pryhuber, G S
AU  - Pryhuber GS
AD  - Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, 
      Ohio 45229-3039.
FAU - Church, S L
AU  - Church SL
FAU - Kroft, T
AU  - Kroft T
FAU - Panchal, A
AU  - Panchal A
FAU - Whitsett, J A
AU  - Whitsett JA
LA  - eng
GR  - HL-38859/HL/NHLBI NIH HHS/United States
GR  - HL-41496/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Proteolipids)
RN  - 0 (Pulmonary Surfactants)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9002-72-6 (Growth Hormone)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Base Sequence
MH  - Growth Hormone/genetics/metabolism
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes/genetics
MH  - Protein Biosynthesis
MH  - Proteolipids/*genetics/*metabolism
MH  - Pulmonary Surfactants/*genetics/*metabolism
MH  - RNA, Messenger/*physiology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1152/ajplung.1994.267.1.L16 [doi]
PST - ppublish
SO  - Am J Physiol. 1994 Jul;267(1 Pt 1):L16-24. doi: 10.1152/ajplung.1994.267.1.L16.