PMID- 8048546
OWN - NLM
STAT- MEDLINE
DCOM- 19940830
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 267
IP  - 1 Pt 1
DP  - 1994 Jul
TI  - Hydrogen peroxide effects on rat mast cell function.
PG  - L85-93
AB  - Oxidant exposure of the airway mucosa may play a significant role in the 
      pathophysiology of asthma and allergic rhinitis. Mast cells play an important 
      role in asthma, and oxidant exposure has been reported to cause direct mast cell 
      degranulation as well as augment immunoglobulin E (IgE)-mediated responses in 
      vivo. H2O2 is an oxidant generated by inflammatory cells and by the interaction 
      of ozone with lipids or aqueous solutions. In this study, the RBL-2H3 mast cell 
      line was used to investigate the ability of H2O2 to induce mast cell responses as 
      well as to effect mast cell responses to IgE and the calcium ionophore A23187. 
      Although cytotoxicity of RBL-2H3 cells at the membrane level was not observed 
      with any concentration of H2O2, DNA damage resulted from exposure to 0.2 and 2.0 
      mM H2O2, and cell proliferation was inhibited by 0.075-0.2 mM H2O2. RBL cell 
      prostaglandin D2 generation was enhanced after 60- and 120-min exposure to 0.2-20 
      mM H2O2. Direct serotonin release required 120-min exposures to 2.0 mM and 60-min 
      exposures to 20 mM H2O2. However, degranulation responses induced by either IgE 
      or A23178 were diminished after exposure to 0.2-2.0 mM H2O2. Lesser amounts 
      (0.005-0.02 mM) had no effect on mast cell function. In summary, H2O2-induced 
      responses of RBL cells, as well as modification of responses to IgE and A23187, 
      occurred only at high concentrations of H2O2, which also induced both 
      intracellular damage and inhibition of cell proliferation. Concentrations of H2O2 
      more likely to be physiologically relevant had no effect on mast cell responses 
      or cytotoxicity.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Peden, D B
AU  - Peden DB
AD  - Department of Pediatrics, School of Medicine, University of North Carolina at 
      Chapel Hill 27599.
FAU - Dailey, L
AU  - Dailey L
FAU - DeGraff, W
AU  - DeGraff W
FAU - Mitchell, J B
AU  - Mitchell JB
FAU - Lee, J G
AU  - Lee JG
FAU - Kaliner, M A
AU  - Kaliner MA
FAU - Hohman, R J
AU  - Hohman RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0R0008Q3JB (Chromium)
RN  - 333DO1RDJY (Serotonin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 37H9VM9WZL (Calcimycin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - RXY07S6CZ2 (Prostaglandin D2)
SB  - IM
MH  - Animals
MH  - Calcimycin/pharmacology
MH  - Cell Degranulation/drug effects
MH  - Cell Survival/drug effects
MH  - Chromium/metabolism
MH  - Colony-Forming Units Assay
MH  - Hydrogen Peroxide/*pharmacology
MH  - Immunoglobulin E/physiology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Mast Cells/*drug effects/metabolism/physiology
MH  - Prostaglandin D2/biosynthesis
MH  - Rats
MH  - Serotonin/metabolism
MH  - Tumor Cells, Cultured
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
AID - 10.1152/ajplung.1994.267.1.L85 [doi]
PST - ppublish
SO  - Am J Physiol. 1994 Jul;267(1 Pt 1):L85-93. doi: 10.1152/ajplung.1994.267.1.L85.